Identification of myogenic-endothelial progenitor cells in the interstitial spaces of skeletal muscle.
Identification of myogenic-endothelial progenitor cells in the interstitial spaces of skeletal muscle.
复制标题
DOI：
10.1083/jcb.200112106
复制
发表时间：
2002-05-13
期刊：
影响因子：
--
通讯作者：
Edgerton VR
中科院分区：
文献类型：
--
作者：
Tamaki T;Akatsuka A;Ando K;Nakamura Y;Matsuzawa H;Hotta T;Roy RR;Edgerton VR
通过免疫组织化学和免疫电镜使用CD 34抗原在小鼠骨骼肌的间质空间中鉴定了推定的肌源性和内皮（肌内皮）细胞祖细胞。基于细胞表面抗原表达，通过荧光激活细胞分选对酶促分离的细胞进行表征，并将其分选为CD 34+和CD 45 −级分。该级分中的细胞对Sca-1为约94%阳性，而对CD 14、31、49、144、c-kit和FLK-1大多为阴性（<3%阳性）。CD 34 +/45−细胞在克隆细胞培养物中形成集落，集落形成单位显示出分化为脂肪细胞、内皮细胞和肌源性细胞的潜力。移植后，CD 34 +/45−细胞在体内完全分化为血管内皮细胞和骨骼肌纤维。分选后，CD 34 +/45−细胞仅表达c-met mRNA，不表达任何其他肌细胞相关标志物，如MyoD、myf-5、myf-6、肌细胞生成素、M-cadherin、Pax-3和Pax-7。然而，培养3天后，这些细胞表达所有肌原性标志物的mRNA。CD 34 +/45−细胞与卫星细胞不同，因为它们表达Bcrp 1/ABCG 2基因mRNA。这些发现表明，肌内皮祖细胞存在于哺乳动物骨骼肌的间隙空间，并且它们可能有助于出生后骨骼肌的生长。
Putative myogenic and endothelial (myo-endothelial) cell progenitors were identified in the interstitial spaces of murine skeletal muscle by immunohistochemistry and immunoelectron microscopy using CD34 antigen. Enzymatically isolated cells were characterized by fluorescence-activated cell sorting on the basis of cell surface antigen expression, and were sorted as a CD34+ and CD45− fraction. Cells in this fraction were ∼94% positive for Sca-1, and mostly negative (<3% positive) for CD14, 31, 49, 144, c-kit, and FLK-1. The CD34+/45− cells formed colonies in clonal cell cultures and colony-forming units displayed the potential to differentiate into adipocytes, endothelial, and myogenic cells. The CD34+/45− cells fully differentiated into vascular endothelial cells and skeletal muscle fibers in vivo after transplantation. Immediately after sorting, CD34+/45− cells expressed only c-met mRNA, and did not express any other myogenic cell-related markers such as MyoD, myf-5, myf-6, myogenin, M-cadherin, Pax-3, and Pax-7. However, after 3 d of culture, these cells expressed mRNA for all myogenic markers. CD34+/45− cells were distinct from satellite cells, as they expressed Bcrp1/ABCG2 gene mRNA. These findings suggest that myo-endothelial progenitors reside in the interstitial spaces of mammalian skeletal muscles, and that they can potentially contribute to postnatal skeletal muscle growth.
登录
查看更多内容
DOI：
10.1083/jcb.150.5.1085
发表时间：
2000-09-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lee JY;Qu-Petersen Z;Cao B;Kimura S;Jankowski R;Cummins J;Usas A;Gates C;Robbins P;Wernig A;Huard J
通讯作者：
Huard J
影响因子：
56.9
作者：
Asahara, T;Murohara, T;Isner, JM
通讯作者：
Isner, JM
DOI：
10.1046/j.1525-1373.1999.d01-55.x
发表时间：
1999-05-01
影响因子：
--
作者：
Young, HE;Steele, TA;Black, AC
通讯作者：
Black, AC
影响因子：
7.8
作者：
De Angelis, L;Berghella, L;Coletta, M;Lattanzi, L;Zanchi, M;Cusella-De Angelis, MG;Ponzetto, C;Cossu, G
通讯作者：
Cossu, G
影响因子：
2.7
作者：
Cornelison, DDW;Wold, BJ
通讯作者：
Wold, BJ