A mini-microscope for in situ monitoring of cells.
A mini-microscope for in situ monitoring of cells.
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DOI：
10.1039/c2lc40345e
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发表时间：
2012-10-21
期刊：
影响因子：
6.1
通讯作者：
Khademhosseini A
中科院分区：
文献类型：
--
作者：
Kim SB;Koo KI;Bae H;Dokmeci MR;Hamilton GA;Bahinski A;Kim SM;Ingber DE;Khademhosseini A
微型显微镜是通过修改现成的商业网络摄像头组件开发的细胞原位监测。微型显微镜由一个CMOS成像模块、一个小塑料透镜和一个白光LED光源组成。CMOS成像模块通过USB端口连接到笔记本电脑上进行图像采集和分析。由于其紧凑的尺寸，8 × 10 × 9厘米，目前的显微镜是便携式的，可以很容易地安装在一个传统的培养箱，并能够实时监测细胞的行为。此外，微型显微镜可用于成像细胞在传统的细胞培养瓶，如皮氏培养皿和多孔板。为了演示微型显微镜的操作，我们在含有三种不同浓度的胎牛血清（5%、10%和20%）的培养基中监测小鼠3T3成纤维细胞的细胞迁移，并证明了血清水平不同的差异反应。此外，我们将胚胎干细胞植入聚乙二醇微孔中，并使用微型显微镜实时监测干细胞聚集体的形成。此外，我们还将用于微液滴生成和分析的片上微流控装置与微型显微镜相结合，观察了不同流动条件下微液滴的形成情况。考虑到其成本效益、强大的成像能力和便携性，该平台可用于低成本的芯片实验室设备实时蜂窝成像的一系列应用。
A mini-microscope was developed for in situ monitoring of cells by modifying off-the-shelf components of a commercial webcam. The mini-microscope consists of a CMOS imaging module, a small plastic lens and a white LED illumination source. The CMOS imaging module was connected to a laptop computer through a USB port for image acquisition and analysis. Due to its compact size, 8 × 10 × 9 cm, the present microscope is portable and can easily fit inside a conventional incubator, and enables real-time monitoring of cellular behaviour. Moreover, the mini-microscope can be used for imaging cells in conventional cell culture flasks, such as Petri dishes and multi-well plates. To demonstrate the operation of the mini-microscope, we monitored the cellular migration of mouse 3T3 fibroblasts in a scratch assay in medium containing three different concentrations of fetal bovine serum (5, 10, and 20%) and demonstrated differential responses depending on serum levels. In addition, we seeded embryonic stem cells inside poly(ethylene glycol) microwells and monitored the formation of stem cell aggregates in real time using the mini-microscope. Furthermore, we also combined a lab-on-a-chip microfluidic device for microdroplet generation and analysis with the mini-microscope and observed the formation of droplets under different flow conditions. Given its cost effectiveness, robust imaging and portability, the presented platform may be useful for a range of applications for real-time cellular imaging using lab-on-a-chip devices at low cost.
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影响因子：
6.1
作者：
Seo S;Su TW;Tseng DK;Erlinger A;Ozcan A
通讯作者：
Ozcan A
影响因子：
3.8
作者：
Su, Ting-Wei;Seo, Sungkyu;Erlinger, Anthony;Ozean, Aydogan
通讯作者：
Ozean, Aydogan
影响因子：
6.1
作者：
Bishara W;Sikora U;Mudanyali O;Su TW;Yaglidere O;Luckhart S;Ozcan A
通讯作者：
Ozcan A
影响因子：
7.4
作者：
Selimovic, Seila;Sim, Woo Young;Kirn, Sang Bok;Jang, Yun Ho;Lee, Won Gu;Khabiry, Masoud;Bae, Hojae;Jambovane, Sachin;Hong, Jong Wook;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
DOI：
10.1073/pnas.0804612105
发表时间：
2008-08-05
影响因子：
11.1
作者：
Cui, Xiquan;Lee, Lap Man;Yang, Changhuei
通讯作者：
Yang, Changhuei