Use of purified F1845 fimbrial adhesin to study localization and expression of receptors for diffusely adhering Escherichia coli during enterocytic differentiation of human colon carcinoma cell lines HT-29 and Caco-2 in culture
Use of purified F1845 fimbrial adhesin to study localization and expression of receptors for diffusely adhering Escherichia coli during enterocytic differentiation of human colon carcinoma cell lines HT-29 and Caco-2 in culture
复制标题
使用纯化的 F1845 菌毛粘附素研究人结肠癌细胞系 HT-29 和 Caco-2 肠细胞分化过程中广泛粘附大肠杆菌受体的定位和表达
作者：
S. Kernéis;S. S. Bilge;V. Fourel;G. Chauvière;M. Coconnier;A. Servin
带有F1845粘附因子的全扩散粘附大肠杆菌（DAEC）C1845细胞与分化的人结肠癌细胞系HT-29和Caco-2扩散结合。通过使用针对纯化的菌毛粘附素F1845因子的抗体，通过间接免疫荧光研究了DAEC F1845特异性刷状缘受体在极化的人肠HT-29和Caco-2上皮细胞中的表达。在未分化的肠细胞中检测到低水平的DAEC F1845受体;它们定位于细胞簇中。DAEC F1845受体在分化的HT-29和Caco-2细胞中以高水平表达。DAEC F1845受体在细胞的顶端域中以惊人的高水平表达，并在培养物中的肠细胞分化期间发展，与肠刷状缘水解酶，蔗糖酶-异麦芽糖酶的顶端表达平行。
Whole diffusely adhering Escherichia coli (DAEC) C1845 cells bearing the F1845 adhesive factor bind diffusely to differentiated human colon carcinoma cell lines HT-29 and Caco-2. By using antibodies directed against the purified fimbrial adhesin F1845 factor, the expression of the DAEC F1845-specific brush border receptors in the polarized human intestinal HT-29 and Caco-2 epithelial cells was studied by indirect immunofluorescence. A low level of DAEC F1845 receptors in undifferentiated intestinal cells was detected; they were localized in a cluster of cells. DAEC F1845 receptors were expressed at a high level in differentiated HT-29 and Caco-2 cells. DAEC F1845 receptors were expressed at a strikingly high level in the apical domains of the cells and developed during enterocytic differentiation in culture, in parallel with the apical expression of the intestinal brush border hydrolase, sucrase-isomaltase.
影响因子：
6.4
作者：
VIAL, PA;ROBINSBROWNE, R;LEVINE, MM
通讯作者：
LEVINE, MM
DOI：
10.1073/pnas.87.20.7839
发表时间：
1990-10-01
影响因子：
11.1
作者：
JERSE, AE;YU, J;KAPER, JB
通讯作者：
KAPER, JB
影响因子：
6.4
作者：
FINLAY, BB;FALKOW, S
通讯作者：
FALKOW, S