Expression of pannexin isoforms in the systemic murine arterial network.
Expression of pannexin isoforms in the systemic murine arterial network.
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DOI：
10.1159/000338758
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发表时间：
2012
影响因子：
1.7
通讯作者：
Isakson BE
中科院分区：
文献类型：
--
作者：
Lohman AW;Billaud M;Straub AC;Johnstone SR;Best AK;Lee M;Barr K;Penuela S;Laird DW;Isakson BE
Pannexins （Panx）形成atp释放通道，并被认为在血管张力的调节中发挥重要作用。然而，Panx在动脉血管中的分布尚无文献记载。我们在转染了编码每个pannexin亚型的质粒和Panx1 - / -小鼠的HEK细胞（不内源性表达Panx蛋白）上测试了针对Panx1， Panx2和Panx3的抗体。每一种Panx抗体都被发现具有特异性，并在分离的动脉上使用免疫细胞化学进行了测试。我们证明Panx1是在动脉网络中检测到的主要亚型。在大动脉中，Panx1主要存在于内皮细胞中，而在小动脉和小动脉中，Panx1主要定位于平滑肌细胞。在冠状动脉中，除了小于100 μm的动脉中可以检测到Panx3外，主要表达Panx1亚型。仅Panx3在球旁器和皮质小动脉中表达。肺动脉和肺泡均有三种Panx亚型的表达。在肌内皮连接处未检测到Panx亚型。我们的结论是，Panx通道在整个脉管系统中的特定局部表达表明，这些通道在调节整个动脉网络中ATP的释放中起着重要作用。
Pannexins (Panx) form ATP-release channels and have been proposed to play an important role in the regulation of vascular tone. However, distribution of Panx across the arterial vasculature is not documented. We tested antibodies against Panx1, Panx2 and Panx3 on HEK cells (which do not endogenously express Panx proteins) transfected with plasmids encoding each pannexin isoform and Panx1−/− mice. Each of the Panx antibodies was found to be specific and was tested on isolated arteries using immunocytochemistry. We demonstrate that Panx1 is the primary isoform detected in the arterial network. In large arteries, Panx1 was primarily in endothelial cells, whereas in small arteries and arterioles Panx1 localizes primarily to the smooth muscle cells. In coronary arteries Panx1 was the predominant isoform expressed, except in arteries less than 100 μm Panx3 became detectable. Only Panx3 was expressed in the juxtaglomerular apparatus and cortical arterioles. The pulmonary artery and alveoli had expression of all three Panx isoforms. No Panx isoforms were detected at the myoendothelial junctions. We conclude that the specific localized expression of Panx channels throughout the vasculature points towards an important role for these channels in regulating the release of ATP throughout the arterial network.
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影响因子：
6.2
作者：
Bond, Stephen R.;Lau, Alice;Naus, Christian C.
通讯作者：
Naus, Christian C.
DOI：
10.1073/pnas.2233464100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Bruzzone, R;Hormuzdi, SG;Monyer, H
通讯作者：
Monyer, H
影响因子：
20.1
作者：
Billaud M;Lohman AW;Straub AC;Looft-Wilson R;Johnstone SR;Araj CA;Best AK;Chekeni FB;Ravichandran KS;Penuela S;Laird DW;Isakson BE
通讯作者：
Isakson BE
影响因子：
7.3
作者：
Buvinic, S;Briones, R;Huidobro-Toro, JP
通讯作者：
Huidobro-Toro, JP
影响因子：
5.5
作者：
FORRESTER, T;LIND, AR
通讯作者：
LIND, AR