Hsc70 is required for endocytosis and clathrin function in Drosophila.
Hsc70 is required for endocytosis and clathrin function in Drosophila.
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DOI：
10.1083/jcb.200205086
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发表时间：
2002-11-11
期刊：
影响因子：
--
通讯作者：
Mellman I
中科院分区：
文献类型：
--
作者：
Chang HC;Newmyer SL;Hull MJ;Ebersold M;Schmid SL;Mellman I
通过筛选果蝇突变体表现出异常的新娘的sevenless（老板）染色模式的眼睛成虫盘上皮细胞，我们已经恢复了Hsc 70 -4，最接近的同源物牛网格蛋白脱壳ATP酶的点突变。虽然突变的等位基因是致命的，FLP/FRT重组产生的突变克隆的分析表明，Sevenless介导的内化的老板在突变Hsc 70 -4眼盘上皮细胞被阻断。其他探针在幼虫加兰细胞中的内吞作用也受到很大抑制。突变细胞的免疫染色和EM分析揭示了内体/溶酶体隔室组织的破坏，包括加兰细胞中网格蛋白包被结构的数量的大幅减少。Hsc 70 -4突变还与发动蛋白的显性负突变体发生遗传相互作用，发动蛋白是网格蛋白包被囊泡（CCV）出芽所需的基因。与这些表型一致，重组突变体Hsc 70蛋白在体外表现出降低的网格蛋白脱壳活性。总之，这些数据提供了遗传支持的长期怀疑的作用Hsc 70网格蛋白介导的内吞作用，至少部分通过抑制CCV的脱壳。
By screening for Drosophila mutants exhibiting aberrant bride of sevenless (Boss) staining patterns on eye imaginal disc epithelia, we have recovered a point mutation in Hsc70-4, the closest homologue to bovine clathrin uncoating ATPase. Although the mutant allele was lethal, analysis of mutant clones generated by FLP/FRT recombination demonstrated that the Sevenless-mediated internalization of Boss was blocked in mutant Hsc70-4 eye disc epithelial cells. Endocytosis of other probes was also greatly inhibited in larval Garland cells. Immunostaining and EM analysis of the mutant cells revealed disruptions in the organization of endosomal/lysosomal compartments, including a substantial reduction in the number of clathrin-coated structures in Garland cells. The Hsc70-4 mutation also interacted genetically with a dominant-negative mutant of dynamin, a gene required for the budding of clathrin-coated vesicles (CCVs). Consistent with these phenotypes, recombinant mutant Hsc70 proteins exhibited diminished clathrin uncoating activity in vitro. Together, these data provide genetic support for the long-suspected role of Hsc70 in clathrin-mediated endocytosis, at least in part by inhibiting the uncoating of CCVs.
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DOI：
10.1083/jcb.131.1.69
发表时间：
1995-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Damke H;Baba T;van der Bliek AM;Schmid SL
通讯作者：
Schmid SL
影响因子：
64.5
作者：
Fölsch, H;Ohno, H;Mellman, I
通讯作者：
Mellman, I
影响因子：
11.4
作者：
Meyer, C;Zizioli, D;Schu, P
通讯作者：
Schu, P
影响因子：
64.5
作者：
Entchev, EV;Schwabedissen, A;González-Gaitán, M
通讯作者：
González-Gaitán, M
影响因子：
64.5
作者：
Hay, BA;Wassarman, DA;Rubin, GM
通讯作者：
Rubin, GM