TfPLC1, a gene encoding phosphoinositide-specific phospholipase C, is predominantly expressed in reproductive organs in Torenia fournieri
TfPLC1, a gene encoding phosphoinositide-specific phospholipase C, is predominantly expressed in reproductive organs in Torenia fournieri
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TfPLC1 是编码磷酸肌醇特异性磷脂酶 C 的基因，主要在蓝猪草的生殖器官中表达
DOI：
10.1007/s00497-008-0081-0
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发表时间：
2008-09
影响因子：
--
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磷脂酰肌醇特异性磷脂酶C(PI-PLC)是一种重要的磷脂酶，在真核细胞信号转导通路中起着关键作用。在植物中，它在生长发育和环境胁迫中起着关键作用。然而，它在有性生殖过程中的信号转导中的作用却知之甚少。在本研究中，我们克隆并鉴定了一个全长PI-PLC基因，命名为TfPLC1。它全长2,171个核苷酸，包括一个开放阅读框，编码一个583个氨基酸的多肽，相对分子质量为66.02 KDa。由该序列推导出的氨基酸序列与其他植物PI-PLC有40%-76%的相似性，并含有特征的X、Y和C2结构域。Northern杂交分析表明，该基因主要在胚珠和花中表达。此外，TfPLC1启动子：：GUS转基因分析表明，它在胚珠、柱头和成熟花粉粒中特异表达。免疫组织化学染色显示，在成熟柱头中，TfPLC1蛋白主要定位于柱头受体面的细胞。综上所述，我们的数据表明TfPLC1可能在植物有性繁殖中发挥重要作用。
Phosphoinositide-specific phospholipase C (PI-PLC) is an important enzyme, which is a key player involved in eukaryotic signal transduction pathways. In plants, it plays a key role in growth and development as well as environmental stress. However, little is known about its roles in signal transduction during sexual reproduction process. In this study, we cloned and characterized a gene of full-length PI-PLC from ovules of Torenia fournieri, designated as TfPLC1. It was 2,171 bp in length, including an open reading frame encoding a polypeptide of 583 amino acids with molecular mass of 66.02 kDa. The amino acid sequence deduced from the cDNA sequence shows 40–76% similarity to other plant PI-PLCs and contains the characteristic X, Y and C2 domains. Northern blot analysis demonstrated it was predominantly expressed in ovules and flowers. Furthermore, TfPLC1 promoter::GUS transgenic analysis indicated it specifically expressed in ovule, stigma and mature pollen grain. Immunohistochemical staining showed that, in mature stigma, TfPLC1 protein was principally localized in the cells of stigmatic receptive surface. Together, our data suggest that TfPLC1 may play an important role in plant sexual reproduction.
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影响因子：
7.2
作者：
Hunt, L;Mills, LN;Gray, JE
通讯作者：
Gray, JE
影响因子：
--
作者：
R. Malhó
通讯作者：
R. Malhó
影响因子：
--
作者：
G. Pfeifer;A. Riggs
通讯作者：
G. Pfeifer;A. Riggs
影响因子：
7.4
作者：
Pina, C;Pinto, F;Becker, JD
通讯作者：
Becker, JD
影响因子：
56.9
作者：
Higashiyama, T;Yabe, S;Kuroiwa, T
通讯作者：
Kuroiwa, T