Lensfree fluorescent on-chip imaging of transgenic Caenorhabditis elegans over an ultra-wide field-of-view.
Lensfree fluorescent on-chip imaging of transgenic Caenorhabditis elegans over an ultra-wide field-of-view.
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DOI：
10.1371/journal.pone.0015955
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发表时间：
2011-01-06
期刊：
影响因子：
3.7
通讯作者：
Ozcan A
中科院分区：
文献类型：
--
作者：
Coskun AF;Sencan I;Su TW;Ozcan A
我们展示了转基因秀丽隐杆线虫（C。elegans）在例如，>2-8 cm 2，空间分辨率为10µm。这是第一次无透镜芯片平台成功成像荧光C。elegans样本在我们的宽视场无透镜成像平台中，转基因样品使用棱镜界面从侧面激发，其中泵浦光通过在基底底面发生的全内反射被拒绝。C.然后将线虫样本记录在FOV上的大面积光电传感器阵列上，>2-8 cm 2，无需使用任何透镜、薄膜干涉滤光片或机械扫描仪。由于荧光发射迅速发散，记录在芯片上的这种无透镜荧光图像由于我们平台的宽点扩散函数而看起来模糊。为了应对这一分辨率挑战，我们使用压缩采样算法将记录的无透镜荧光图案唯一解码为更高分辨率的图像，分辨率为10 μm。我们使用不同类型的光电传感器测试了这种压缩解码方法的有效性，以实现类似的分辨率水平，而不依赖于成像芯片。我们进一步证明，这种宽FOV无透镜荧光成像平台也可以使用部分相干无透镜数字同轴全息术对相同样品进行连续明场成像，该部分相干无透镜数字同轴全息术从荧光激发中使用的相同棱镜的顶面耦合。这种独特的组合允许C的超宽场双模成像。这尤其可以为生物医学研究中的高通量筛选应用提供有用的工具。
We demonstrate lensfree on-chip fluorescent imaging of transgenic Caenorhabditis elegans (C. elegans) over an ultra-wide field-of-view (FOV) of e.g., >2–8 cm2 with a spatial resolution of ∼10µm. This is the first time that a lensfree on-chip platform has successfully imaged fluorescent C. elegans samples. In our wide-field lensfree imaging platform, the transgenic samples are excited using a prism interface from the side, where the pump light is rejected through total internal reflection occurring at the bottom facet of the substrate. The emitted fluorescent signal from C. elegans samples is then recorded on a large area opto-electronic sensor-array over an FOV of e.g., >2–8 cm2, without the use of any lenses, thin-film interference filters or mechanical scanners. Because fluorescent emission rapidly diverges, such lensfree fluorescent images recorded on a chip look blurred due to broad point-spread-function of our platform. To combat this resolution challenge, we use a compressive sampling algorithm to uniquely decode the recorded lensfree fluorescent patterns into higher resolution images, demonstrating ∼10 µm resolution. We tested the efficacy of this compressive decoding approach with different types of opto-electronic sensors to achieve a similar resolution level, independent of the imaging chip. We further demonstrate that this wide FOV lensfree fluorescent imaging platform can also perform sequential bright-field imaging of the same samples using partially-coherent lensfree digital in-line holography that is coupled from the top facet of the same prism used in fluorescent excitation. This unique combination permits ultra-wide field dual-mode imaging of C. elegans on a chip which could especially provide a useful tool for high-throughput screening applications in biomedical research.
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影响因子：
7.4
作者：
Seo, Sungkyu;Isikman, Serhan O.;Sencan, Ikbal;Mudanyali, Onur;Su, Ting-Wei;Bishara, Waheb;Erlinger, Anthony;Ozcan, Aydogan
通讯作者：
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DOI：
10.1109/jstsp.2007.910971
发表时间：
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作者：
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作者：
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