Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis.
Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis.
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腐生的瘦肉纤溶酶的基因组序列提供了对钩端螺旋体进化和钩端螺旋体病的发病机理的见解。
DOI：
10.1371/journal.pone.0001607
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发表时间：
2008-02-13
期刊：
影响因子：
3.7
通讯作者：
Adler, Ben
中科院分区：
文献类型：
--
作者：
Picardeau, Mathieu;Bulach, Dieter M.;Bouchier, Christiane;Zuerner, Richard L.;Zidane, Nora;Wilson, Peter J.;Creno, Sophie;Kuczek, Elizabeth S.;Bommezzadri, Simona;Davis, John C.;McGrath, Annette;Johnson, Matthew J.;Boursaux-Eude, Caroline;Seemann, Torsten;Rouy, Zoe;Coppel, Ross L.;Rood, Julian I.;Lajus, Aurelie;Davies, John K.;Medigue, Claudine;Adler, Ben
双曲钩端螺旋体是存在于水生环境中的自由生活的嗜盐螺旋体。测定了L. biflexa，使其成为第一个被测序的嗜血钩端螺旋体。洛杉矶biflexa基因组有3，590个蛋白质编码基因，分布在三个环状复制子上：主要的3，604染色体，一个较小的278-kb复制子，也携带必需基因，和第三个74-kb复制子。比较序列分析表明L. biflexa是一个很好的模型，钩端螺旋体进化的研究，我们得出结论，2052个基因（61%）代表了祖先的基因组存在之前的致病性和saperitic钩端螺旋体物种的分歧。L. biflexa基因组与两个致病性钩端螺旋体物种揭示了几个主要的发现。近三分之一的L。双曲基因在致病性钩端螺旋体中不存在。我们建议，一旦纳入L。由于高基因密度和有限的转座因子的存在所施加的物理限制，侧向转移的DNA经历最小的重排。相反，致病性钩端螺旋体物种的基因组经历频繁的重排，通常涉及插入序列之间的重组。鉴定了两种致病菌L. borgpetersenii和L.问号，但在L. biflexa与这些基因在发病机制中的作用一致。环境敏感能力的差异。biflexa，L. borgpetersenii和L. Interrogans提出了一个假设L. borgpetersenii在哺乳动物宿主外的存活能力受到影响，而L.问号线虫保留了促进疾病通过水传播的环境感觉功能。
Leptospira biflexa is a free-living saprophytic spirochete present in aquatic environments. We determined the genome sequence of L. biflexa, making it the first saprophytic Leptospira to be sequenced. The L. biflexa genome has 3,590 protein-coding genes distributed across three circular replicons: the major 3,604 chromosome, a smaller 278-kb replicon that also carries essential genes, and a third 74-kb replicon. Comparative sequence analysis provides evidence that L. biflexa is an excellent model for the study of Leptospira evolution; we conclude that 2052 genes (61%) represent a progenitor genome that existed before divergence of pathogenic and saprophytic Leptospira species. Comparisons of the L. biflexa genome with two pathogenic Leptospira species reveal several major findings. Nearly one-third of the L. biflexa genes are absent in pathogenic Leptospira. We suggest that once incorporated into the L. biflexa genome, laterally transferred DNA undergoes minimal rearrangement due to physical restrictions imposed by high gene density and limited presence of transposable elements. In contrast, the genomes of pathogenic Leptospira species undergo frequent rearrangements, often involving recombination between insertion sequences. Identification of genes common to the two pathogenic species, L. borgpetersenii and L. interrogans, but absent in L. biflexa, is consistent with a role for these genes in pathogenesis. Differences in environmental sensing capacities of L. biflexa, L. borgpetersenii, and L. interrogans suggest a model which postulates that loss of signal transduction functions in L. borgpetersenii has impaired its survival outside a mammalian host, whereas L. interrogans has retained environmental sensory functions that facilitate disease transmission through water.
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影响因子：
4.4
作者：
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通讯作者：
Rocap, Gabrielle
影响因子：
3.1
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