Advances in venomics: Modern separation techniques and mass spectrometry.
Advances in venomics: Modern separation techniques and mass spectrometry.
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DOI：
10.1016/j.jchromb.2020.122352
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发表时间：
2020-12-01
期刊：
影响因子：
--
通讯作者：
Stockand JD
中科院分区：
文献类型：
--
作者：
Abd El-Aziz TM;Soares AG;Stockand JD
蛇毒是生物活性蛋白质和非蛋白质成分的复杂化学混合物。毒素具有广泛的靶点和作用，包括离子通道和膜受体，以及血小板聚集和血小板栓形成。毒素以高亲和力和选择性靶向这些效应子和效应。从药理学的角度来看，蛇毒化合物是药物发现和开发的宝贵资源。然而，使用蛇毒进行药物发现的主要挑战是分离和分析这些复杂混合物中的生物活性蛋白质和肽。从复杂的混合物（如蛇毒）中获取分子信息需要蛋白质组学分析，通常与毒腺的转录组学分析相结合。本综述总结了目前的知识，并强调了最近的重要进展，特别强调当代分离技术和生物信息学已经开始阐述蛇毒的复杂性。几种分析技术，如二维凝胶电泳，反相高效液相色谱法，尺寸排阻色谱法，离子交换色谱法，MALDI-TOF-MS，和LC-ESI-QTOF-MS已被用于这方面。分离方法的改进，如多维HPLC，二维电泳耦合软电离（MALDI和ESI）质谱已成为关键，以获得一个惊人的复杂性毒液的准确图片。在生物信息学方面，各种软件工具，如PEAKS也已成功使用。从毒液组学中收集的这些信息对于预测和解决毒液活性成分的生物活性都很重要，这反过来又是基于这些毒液成分开发新药的关键。
Snake venoms are complex chemical mixtures of biologically active proteins and non-protein components. Toxins have a wide range of targets and effects to include ion channels and membrane receptors, and platelet aggregation and platelet plug formation. Toxins target these effectors and effects at high affinity and selectivity. From a pharmacological perspective, snake venom compounds are a valuable resource for drug discovery and development. However, a major challenge to drug discovery using snake venoms is isolating and analyzing the bioactive proteins and peptides in these complex mixtures. Getting molecular information from complex mixtures such as snake venoms requires proteomic analyses, generally combined with transcriptomic analyses of venom glands. The present review summarizes current knowledge and highlights important recent advances in venomics with special emphasis on contemporary separation techniques and bioinformatics that have begun to elaborate the complexity of snake venoms. Several analytical techniques such as two-dimensional gel electrophoresis, RP-HPLC, size exclusion chromatography, ion exchange chromatography, MALDI-TOF-MS, and LC-ESI-QTOF-MS have been employed in this regard. The improvement of separation approaches such as multidimensional-HPLC, 2D-electrophoresis coupled to soft-ionization (MALDI and ESI) mass spectrometry has been critical to obtain an accurate picture of the startling complexity of venoms. In the case of bioinformatics, a variety of software tools such as PEAKS also has been used successfully. Such information gleaned from venomics is important to both predicting and resolving the biological activity of the active components of venoms, which in turn is key for the development of new drugs based on these venom components.
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影响因子：
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DOI：
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发表时间：
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作者：
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