Genomics of Staphylococcus aureus ocular isolates.
Genomics of Staphylococcus aureus ocular isolates.
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DOI：
10.1371/journal.pone.0250975
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Wozniak RAF
中科院分区：
文献类型：
--
作者：
Johnson WL;Sohn MB;Taffner S;Chatterjee P;Dunman PM;Pecora N;Wozniak RAF
金黄色葡萄球菌是眼部感染的主要原因，通常会导致毁灭性的视力丧失。尽管与这些感染相关的发病率很高，但关于可能对眼组织有偏好的特定菌株类型，以及在这一特定生物利基中驱动其致病性的毒力因子集，人们迄今知之甚少。全基因组测序(WGS)可以提供对临床分离株特定亚群中导致疾病的菌株类型和毒力因素的前瞻性、全面的评估，从而在这方面提供有价值的见解。因此，对一组163个成员的金黄色葡萄球菌眼部临床菌株进行了测序，并以高通量的方式评估了与眼部感染相关的常见菌株类型(多位点序列类型(MLST)、SpA、AGR)以及235个已知毒力因子的存在/不存在。然后将该眼部菌株集与NCBI整理的116个成员的非眼部金黄色葡萄球菌全序列集进行直接比较，以确定眼部和非眼部金黄色葡萄球菌分离株之间的关键差异。在眼部金黄色葡萄球菌分离株中发现的最常见的序列类型是ST5、ST8和ST30，通常反映了循环中的非眼部致病性金黄色葡萄球菌菌株。然而，重要的是，眼部分离株被发现显著富含一组肠毒素，这表明这类毒力因子在促进眼部疾病方面具有潜在的作用。进一步的基因组分析表明，这些肠毒素位于可移动的致病岛上，因此水平基因转移可能促进肠毒素的获得，从而有可能放大金黄色葡萄球菌在眼组织中的毒力。
Staphylococcus aureus is a major cause of ocular infections, often resulting in devastating vision loss. Despite the significant morbidity associated with these infections, little is yet known regarding the specific strain types that may have a predilection for ocular tissues nor the set of virulence factors that drive its pathogenicity in this specific biological niche. Whole genome sequencing (WGS) can provide valuable insight in this regard by providing a prospective, comprehensive assessment of the strain types and virulence factors driving disease among specific subsets of clinical isolates. As such, a set of 163-member S. aureus ocular clinical strains were sequenced and assessed for both common strain types (multilocus sequence type (MLST), spa, agr) associated with ocular infections as well as the presence/absence of 235 known virulence factors in a high throughput manner. This ocular strain set was then directly compared to a fully sequenced 116-member non-ocular S. aureus strain set curated from NCBI in order to identify key differences between ocular and non-ocular S. aureus isolates. The most common sequence types found among ocular S. aureus isolates were ST5, ST8 and ST30, generally reflecting circulating non-ocular pathogenic S. aureus strains. However, importantly, ocular isolates were found to be significantly enriched for a set of enterotoxins, suggesting a potential role for this class of virulence factors in promoting ocular disease. Further genomic analysis revealed that these enterotoxins are located on mobile pathogenicity islands, thus horizontal gene transfer may promote the acquisition of enterotoxins, potentially amplifying S. aureus virulence in ocular tissues.
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影响因子：
5.2
作者：
Asadollahi P;Farahani NN;Mirzaii M;Khoramrooz SS;van Belkum A;Asadollahi K;Dadashi M;Darban-Sarokhalil D
通讯作者：
Darban-Sarokhalil D
影响因子：
5.2
作者：
Challagundla L;Reyes J;Rafiqullah I;Sordelli DO;Echaniz-Aviles G;Velazquez-Meza ME;Castillo-Ramírez S;Fittipaldi N;Feldgarden M;Chapman SB;Calderwood MS;Carvajal LP;Rincon S;Hanson B;Planet PJ;Arias CA;Diaz L;Robinson DA
通讯作者：
Robinson DA
影响因子：
4.2
作者：
Hsu HY;Ernst B;Schmidt EJ;Parihar R;Horwood C;Edelstein SL
通讯作者：
Edelstein SL
DOI：
10.1007/bf01586186
发表时间：
1996-01-01
影响因子：
4.5
作者：
Frenay, HME;Bunschoten, AE;Mooi, FR
通讯作者：
Mooi, FR
影响因子：
9.4
作者：
Bartels, Mette Damkjaer;Petersen, Andreas;Westh, Henrik
通讯作者：
Westh, Henrik