Epigenomic consequences of immortalized plant cell suspension culture.
Epigenomic consequences of immortalized plant cell suspension culture.
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DOI：
10.1371/journal.pbio.0060302
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发表时间：
2008-12-09
期刊：
影响因子：
9.8
通讯作者：
Martienssen RA
中科院分区：
文献类型：
--
作者：
Tanurdzic M;Vaughn MW;Jiang H;Lee TJ;Slotkin RK;Sosinski B;Thompson WF;Doerge RW;Martienssen RA
培养物中生长的植物细胞表现出遗传和表观遗传不稳定性。使用相结合的染色质免疫沉淀和DNA甲基化分析平铺微阵列，我们已经映射的位置和丰富的组蛋白和DNA修饰在一个连续增殖，去分化的细胞悬浮培养的拟南芥。我们发现常染色质在培养物中变得高度甲基化，并且一小部分高度甲基化的基因与异染色质标记相关。与此相反，异染色质经历戏剧性的和非常精确的DNA低甲基化与特定的转座因子（TE）在文化中的转录激活。小干扰RNA（siRNA）的高通量测序显示，在培养物中活化的TE具有增加的21-核苷酸（nt）siRNA水平，有时以24-nt siRNA类为代价。相比之下，与主要的24-nt siRNA类匹配的保持沉默的TE在其siRNA谱中没有显著变化。这些结果暗示RNA干扰和染色质修饰在永生化细胞培养物中TE激活后基因组的表观遗传重组中。培养的植物细胞在遗传和表观遗传上都是不稳定的。我们研究了长期植物细胞培养的表观基因组后果，发现一些基因显示出DNA甲基化的增加，这让人想起永生化的动物细胞系和癌细胞。相比之下，在基因组的异染色质部分，一些转座因子（TE）经历DNA甲基化和转录激活的戏剧性和非常精确的损失。在培养物中再活化的TE也伴随着21个核苷酸（nt）的小RNA的产生。相反，保持甲基化和沉默的TE保留了24-nt小RNA的主要类别，并且其小RNA谱没有显著变化。我们的研究结果表明，在永生化细胞培养物中TE激活后，RNA干扰参与了基因组的表观遗传重组。永生化植物细胞经历表观基因组变化，使人想起永生化动物细胞系和癌细胞，伴随着小RNA类别的转变，暗示RNAi机制在调节表观基因组中的作用。
Plant cells grown in culture exhibit genetic and epigenetic instability. Using a combination of chromatin immunoprecipitation and DNA methylation profiling on tiling microarrays, we have mapped the location and abundance of histone and DNA modifications in a continuously proliferating, dedifferentiated cell suspension culture of Arabidopsis. We have found that euchromatin becomes hypermethylated in culture and that a small percentage of the hypermethylated genes become associated with heterochromatic marks. In contrast, the heterochromatin undergoes dramatic and very precise DNA hypomethylation with transcriptional activation of specific transposable elements (TEs) in culture. High throughput sequencing of small interfering RNA (siRNA) revealed that TEs activated in culture have increased levels of 21-nucleotide (nt) siRNA, sometimes at the expense of the 24-nt siRNA class. In contrast, TEs that remain silent, which match the predominant 24-nt siRNA class, do not change significantly in their siRNA profiles. These results implicate RNA interference and chromatin modification in epigenetic restructuring of the genome following the activation of TEs in immortalized cell culture. Cultured plant cells are genetically and epigenetically unstable. We investigated the epigenomic consequences of long-term plant cell culture and found that some genes show an increase in DNA methylation, reminiscent of immortalized animal cell lines and cancer cells. By contrast, in the heterochromatic portion of the genome, some transposable elements (TE), undergo dramatic and very precise loss of DNA methylation and transcriptional activation. The reactivated TEs in culture are also accompanied by a production of 21-nucleotide (nt) small RNAs. In contrast, TEs that remain methylated and silent retain the predominant class of 24-nt small RNAs, and do not change significantly in their small RNA profiles. Our results implicate RNA interference in epigenetic restructuring of the genome following the activation of TEs in immortalized cell culture. Immortalized plant cells undergo epigenomic changes, reminiscent of immortalized animal cell lines and cancer cells, accompanied by shifts in small RNA classes, implicating a role for the RNAi machinery in regulating the epigenome.
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影响因子：
2.6
作者：
Fransz, P;ten Hoopen, R;Tessadori, F
通讯作者：
Tessadori, F
影响因子：
1.5
作者：
DIFRANCO, C;PISANO, C;JUNAKOVIC, N
通讯作者：
JUNAKOVIC, N
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
5.4
作者：
BROWN, PTH;GOBEL, E;LORZ, H
通讯作者：
LORZ, H
DOI：
10.1007/bf00337777
发表时间：
1987-11-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
DENNIS, ES;BRETTELL, RIS;PEACOCK, WJ
通讯作者：
PEACOCK, WJ