Reduced expression of syndecan-1 affects metastatic potential and clinical outcome in patients with colorectal cancer.
Reduced expression of syndecan-1 affects metastatic potential and clinical outcome in patients with colorectal cancer.
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DOI：
10.1111/j.1349-7006.2001.tb01062.x
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发表时间：
2001-10
期刊：
影响因子：
--
通讯作者：
Kohgo Y
中科院分区：
文献类型：
--
作者：
Fujiya M;Watari J;Ashida T;Honda M;Tanabe H;Fujiki T;Saitoh Y;Kohgo Y
Syndecan-1是一种跨膜硫酸乙酰肝素蛋白聚糖，可调节细胞与细胞或细胞与细胞外基质的相互作用，并可能影响恶性细胞行为。我们研究了syndecan-1在结直肠癌中的表达变化，并分析了组织学和临床特征之间的关系。采用免疫组织化学染色法检测结直肠癌组织中Syndecan-1蛋白的表达。使用syndecan-1核糖探针进行原位杂交以确认转录信号。还通过定量PCR分析了在有或没有甲基化抑制剂的情况下培养的癌细胞系中的多配体蛋白聚糖-1 mRNA表达。在检测的105份标本中，36份（34.3%）中不到25%的肿瘤细胞被抗syndecan-1单克隆抗体染色。原位杂交显示了类似的染色轮廓，免疫组织化学。甲基化抑制剂5-aza-2′-deoxycytidine可增加Syndecan-1 mRNA的表达，表明高甲基化参与了syndecan-1表达的抑制。临床上，syndecan-1阴性肿瘤患者的淋巴结或肝脏转移发生率显著较高。在表现出原发性浸润期的T1结直肠癌中，淋巴结转移、未分化特征和癌细胞的“出芽”在syndecan-1阴性肿瘤中更常见。syndecan-1阴性肿瘤患者的生存率以不依赖于分期的方式显著降低。这些结果表明，结直肠癌细胞中syndecan-1表达的减少，这应该是在转录水平上调节的，与侵袭性密切相关。syndecan-1在结直肠癌中表达的评估可以预测患者术后的生存率
Syndecan‐1 is a transmembrane heparansulfate proteoglycan which regulates cell‐to‐cell or cell‐to‐extracellular matrix interactions and may influence malignant cell behavior. We investigated the alterations of syndecan‐1 expressions in colorectal cancers and analyzed the relationship between histological and clinical characteristics. Syndecan‐1 protein expression in colorectal cancer tissues was investigated with immunohistochemical staining of resected specimens. In situ hybridization was performed using syndecan‐1 riboprobe to confirm the transcriptional signals. Syndecan‐1 mRNA expression in cancer cell lines cultured with or without methylation inhibitor was also analyzed by quantitative PCR. Out of 105 specimens tested, less than 25% of tumor cells were stained with anti‐syndecan‐1 monoclonal antibody in 36 (34.3%). In situ hybridization showed a similar staining profile to that of immunohistochemistry. Syndecan‐1 mRNA expression was increased by the methylation inhibitor 5‐aza‐2′‐deoxycytidine, suggesting that the hypermethylation is involved in the suppression of syndecan‐1 expression. Clinically, the incidence of metastasis to lymphnode or liver in patients with syndecan‐1‐negative tumors was significantly high. Among Tl colorectal cancers displaying a primary invasive phase, lymphnode metastasis, undifferentiated characters and ‘budding’ of cancer cells were more common in syndecan‐1‐negative tumors. The survival rate in patients with syndecan‐1‐negative tumors was decreased significantly in a stage‐independent manner. These results suggest that the reduction of syndecan‐1 expression in colorectal cancer cells, which is supposed to be regulated at the transcription level, is closely related to invasive character. The evaluation of syndecan‐1 expression in colorectal cancer may allow prediction of patients' survival after surgery
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富含脯氨酸的抗微生物肽，PR-39基因转导改变了人肝细胞癌细胞中的侵入性活性和肌动蛋白结构。
影响因子：
8.8
作者：
Ohtake, T;Fujimoto, Y;Ikuta, K;Saito, H;Ohhira, M;Ono, M;Kohgo, Y
通讯作者：
Kohgo, Y
影响因子：
4.8
作者：
Volk, R;Schwartz, JJ;Simons, M
通讯作者：
Simons, M
DOI：
10.1073/pnas.91.23.11035
发表时间：
1994-11-08
影响因子：
11.1
作者：
GALLO, RL;ONO, M;BERNFIELD, M
通讯作者：
BERNFIELD, M
影响因子：
45.3
作者：
Goldberg, RM;Hatfield, AK;Moertel, CG
通讯作者：
Moertel, CG
影响因子：
13.5
作者：
ROSKAMS, T;MOSHAGE, H;DESMET, V
通讯作者：
DESMET, V