Membrane tension increases fusion efficiency of model membranes in the presence of SNAREs.
Membrane tension increases fusion efficiency of model membranes in the presence of SNAREs.
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DOI：
10.1038/s41598-017-12348-w
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发表时间：
2017-09-21
影响因子：
4.6
通讯作者：
Janshoff A
中科院分区：
文献类型：
--
作者：
Kliesch TT;Dietz J;Turco L;Halder P;Polo E;Tarantola M;Jahn R;Janshoff A
在生物系统中神经递质释放期间发生的膜融合与模型膜之间观察到的融合之间的时间尺度上的巨大差距引起了对大量可能解释这种差异的可能因素的猜测。一个可能的原因是突触前膜的侧膜张力升高。我们研究了张力依赖性的融合模型膜配备了一个最小的融合机制，包括syntaxin 1，synaptobrevin和SNAP 25。实现了两种不同的策略;一种是基于支持的双层，另一种是采用无座巨型脂质体。在第一种方法中，将来自含有突触融合蛋白1和预组装SNAP 25（Δ N-复合物）的巨大单层囊泡的平面双层的孤立补丁沉积在可折叠的PDMS片上。在第二种方法中，通过在功能化表面上粘附完整的巨大单层囊泡来控制侧膜张力。在这两种方法中，聚变效率随着横向张力的增加而显著增加，我们确定了3.4 mN m−1的阈值张力，在该阈值张力下，聚变事件的数量大幅增加。
The large gap in time scales between membrane fusion occurring in biological systems during neurotransmitter release and fusion observed between model membranes has provoked speculations over a large number of possible factors that might explain this discrepancy. One possible reason is an elevated lateral membrane tension present in the presynaptic membrane. We investigated the tension-dependency of fusion using model membranes equipped with a minimal fusion machinery consisting of syntaxin 1, synaptobrevin and SNAP 25. Two different strategies were realized; one based on supported bilayers and the other one employing sessile giant liposomes. In the first approach, isolated patches of planar bilayers derived from giant unilamellar vesicles containing syntaxin 1 and preassembled SNAP 25 (ΔN-complex) were deposited on a dilatable PDMS sheet. In a second approach, lateral membrane tension was controlled through the adhesion of intact giant unilamellar vesicles on a functionalized surface. In both approaches fusion efficiency increases considerably with lateral tension and we identified a threshold tension of 3.4 mN m−1, at which the number of fusion events is increased substantially.
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影响因子：
5.4
作者：
Paluch EK;Nelson CM;Biais N;Fabry B;Moeller J;Pruitt BL;Wollnik C;Kudryasheva G;Rehfeldt F;Federle W
通讯作者：
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通讯作者：
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影响因子：
3.4
作者：
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通讯作者：
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