The apoplastic oxidative burst as a key factor of hyperhydricity in garlic plantlet in vitro
The apoplastic oxidative burst as a key factor of hyperhydricity in garlic plantlet in vitro
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质外体氧化爆发是大蒜试管苗体内水分过多的关键因素
DOI：
10.1007/s11240-014-0623-0
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发表时间：
2015-02
影响因子：
3
通讯作者：
Wu Zhen
中科院分区：
文献类型：
--
作者：
Tian Jie;Jiang Fangling;Wu Zhen
水分过多现象是组织培养中经常发生的一种生理障碍，会导致芽的超微结构改变和代谢改变。活性氧（ROS）积累和氧化应激诱导是水过多发生过程中的常见特征，但细胞器氧化还原稳态和水过多与超微结构异常之间的关系尚不清楚。为了研究氧化应激诱导的水分过多的起源，研究了体外大蒜苗不同亚细胞区室中氧代谢的变化。在外源过氧化氢（H2O2）胁迫下，叶绿体和线粒体超微结构被破坏，伴随着水分过多的频率和严重程度的加剧。向生长培养基中添加 H2O2 增强了亚细胞区室中超氧阴离子的产生和 H2O2 含量。质外体中ROS的积累最高。与对照芽相比，在质外体中，外源 H2O2 在 4 天内刺激超氧化物歧化酶活性急剧增加，在 H2O2 处理 8 天后，抗坏血酸过氧化物酶和谷胱甘肽还原酶活性以及抗坏血酸和谷胱甘肽含量急剧增加。在其他亚细胞区室中，12 天后抗氧化系统发生显着改善。因此，质外体是所研究的区室中最敏感的区室。质外体活性氧可能发挥信号作用，参与应激适应的协调。大蒜试管苗中的质外体氧化爆发是对多水性发展的早期反应。
The phenomenon of hyperhydricity, a physiological disorder occurring frequently in tissue culture, causes ultrastructural modification and metabolic alteration of shoots. Reactive oxygen species (ROS) accumulation and oxidative stress induction are common features during the development of hyperhydricity, but the relationship between organelle redox homeostasis and hyperhydricity with ultrastructural abnormalities is unclear. To investigate the origin of oxidative stress-induced hyperhydricity, changes in oxygen metabolism in different subcellular compartments of garlic plantlets in vitro were studied. Under exogenous hydrogen peroxide (H2O2) stress, the chloroplastic and mitochondrial ultrastructure was disrupted, which was concomitant with aggravated frequency and severity of hyperhydricity. The addition of H2O2to the growth medium enhanced superoxide anion generation and H2O2content in the subcellular compartments. Accumulation of ROS was the highest in apoplasts. Compared with control shoots, in apoplasts exogenous H2O2stimulated a sharp increase in superoxide dismutase activity within 4 days and a sharp increase in ascorbate peroxidase and glutathione reductase activities and in ascorbic acid and glutathione contents after 8 days of H2O2treatment. In the other subcellular compartments, dramatic improvement of the antioxidant system occurred after 12 days. Thus, the apoplast was the most sensitive compartment among those investigated. Apoplastic ROS might play a signaling role to participate in the coordination of stress adaptation. The apoplastic oxidative burst in garlic plantlets in vitro is an early response to the development of hyperhydricity.
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DOI：
10.1042/bj20081386
发表时间：
2009-01-01
期刊：
The Biochemical journal
影响因子：
--
作者：
Murphy MP
通讯作者：
Murphy MP
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
Michael Wrzaczek;M. Brosché;J. Salojärvi;Saijaliisa Kangasjärvi;N. Idänheimo;Sophia Mersmann;S. Robatzek-S.-Robatz
通讯作者：
Michael Wrzaczek;M. Brosché;J. Salojärvi;Saijaliisa Kangasjärvi;N. Idänheimo;Sophia Mersmann;S. Robatzek-S.-Robatz
影响因子：
--
作者：
Leadsham JE;Gourlay CW
通讯作者：
Gourlay CW
水分胁迫下玉米叶片不同亚细胞区室抗氧化（同）酶和抗氧化剂的分布与配合
影响因子：
4.8
作者：
Zhang Aying
通讯作者：
Zhang Aying
影响因子：
6.6
作者：
M. Schwarzländer;I. Finkemeier
通讯作者：
M. Schwarzländer;I. Finkemeier