On-chip integration of normal phase high-performance liquid chromatography and droplet microfluidics introducing ethylene glycol as polar continuous phase for the compartmentalization of n-heptane eluents.
On-chip integration of normal phase high-performance liquid chromatography and droplet microfluidics introducing ethylene glycol as polar continuous phase for the compartmentalization of n-heptane eluents.
复制标题
正相高效液相色谱和液滴微流体的芯片集成，引入乙二醇作为极性连续相，用于正庚烷洗脱液的区室化
DOI：
10.1016/j.chroma.2019.460653
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
D. Belder
中科院分区：
文献类型：
--
作者：
A. J. Peretzki;D. Belder
介绍了一种集成芯片的正相液相色谱柱后分割方法。这是通过在单个微流体玻璃芯片中无缝集成手性NP芯片HPLC柱、流动聚焦液滴发生器和分段流动通道来实现的。这允许将洗脱液连续分割成液滴，所述液滴通过不混溶的连续相被拾取和输送。NP-芯片-HPLC和液滴微流体技术的组合能够在皮升规模下为进一步的下游工艺进行分离和保存色谱运行。一个重要方面是适当选择连续相的极性、润湿性和粘度。为此目的，引入乙二醇，这有助于正相色谱和液滴微流体的第一次组合。通过调节流速和改变发生器的几何形状，可以精确地控制液滴的大小和频率。
We introduce an integrated chip-approach for a postcolumn segmentation of normal phase liquid chromatography. This is achieved by the seamless integration of a chiral NP-chip-HPLC column, a flow-focusing droplet generator, and a segmented flow channel in a single microfluidic glass chip. This allows a continuous segmentation of the eluent into droplets which are picked up and transported via an immiscible continuous phase. The combination of NP-chip-HPLC and droplet microfluidics enables to fractionate and conserve chromatographic runs for further downstream processes at picoliter scale. An essential aspect is the proper choice of the continuous phase concerning polarity, wetting properties and viscosity. For this purpose, ethylene glycol is introduced which facilitates this first combination of normal phase chromatography and droplet microfluidics. By adjusting the flow rates and varying the generator geometry, the size and frequency of the droplets could be precisely controlled.
登录
查看更多内容
将单片多孔聚合物与液滴基于基于液滴的微流体的集成在芯片上进行纳米/picoliter体积样品分析。
影响因子：
11.7
作者：
Kim JY;Chang SI;deMello AJ;O'Hare D
通讯作者：
O'Hare D
影响因子：
7.4
作者：
Lotter, Carsten;Heiland, Josef J.;Belder, Detlev
通讯作者：
Belder, Detlev
DOI：
10.1016/j.trac.2015.08.002
发表时间：
2016-07
期刊：
Trends in analytical chemistry : TRAC
影响因子：
--
作者：
Grinias JP;Kennedy RT
通讯作者：
Kennedy RT
用于高通量正相手性分析的多并行微流控高效液相色谱。
影响因子：
4.1
作者：
P. Sajonz;W. Schafer;Xiaoyi Gong;S. Shultz;T. Rosner;C. Welch
通讯作者：
C. Welch
影响因子：
7.4
作者：
Li, Qiang;Pei, Jian;Song, Peng;Kennedy, Robert T.
通讯作者：
Kennedy, Robert T.