Budding pathway in the templated assembly of viruslike particles.
Budding pathway in the templated assembly of viruslike particles.
复制标题
病毒样颗粒模板化组装中的出芽途径。
DOI：
--
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发表时间：
2013
影响因子：
3.3
通讯作者：
B. Dragnea
中科院分区：
文献类型：
--
作者：
A. Malyutin;B. Dragnea
通过时程光散射和冷冻电子断层扫描观察到病毒衣壳蛋白围绕无机纳米颗粒核心组装的新途径。平均直径为 11.3 nm 的金纳米颗粒已被用作不同浓度的雀麦花叶病毒 (BMV) 衣壳蛋白组装的模板。至少在低蛋白质浓度下，散射和消光测量的动力学特征与大的纳米颗粒-蛋白质簇的初始快速形成一致，该簇随后分离成单个病毒样颗粒（VLP）。冷冻电镜证实了纳米粒子和蛋白质混合后短时间内多粒子簇的出现。多粒子簇的冷冻电子断层扫描得出平均表面到表面粒子间距离为~7.5 nm，相当于蛋白质壳厚度的~1.5倍。我们提出了一种方案，其中 VLP 的生成可能是通过具有缺陷或不完整壳的聚集颗粒之间的单体交换来发生的，从而导致稳定的二十面体壳的形成，最终从聚集体中萌芽。与之前的工作结果一起，这些发现凸显了植物病毒衣壳蛋白组装的惊人多功能性。这种以前未知的 VLP 形成机制具有可能与细胞内病毒工厂的拥挤环境相关的特征。
A new pathway for the assembly of viral capsid protein around inorganic nanoparticle cores was observed by time-course light scattering and cryo-electron tomography. Gold nanoparticles with an average diameter of 11.3 nm have been used as a template for the assembly of Brome mosaic virus (BMV) capsid protein at different concentrations. At least at low protein concentrations the kinetic features of the scattering and extinction measurements are consistent with the initial rapid formation of large nanoparticle-protein clusters, which subsequently separate into individual viruslike particles (VLPs). The occurrence of multiparticle clusters at short times after mixing nanoparticles and proteins was confirmed by cryo-EM. Cryo-electron tomography of the multiparticle clusters yielded an average surface-to-surface interparticle distance of ∼7.5 nm, equivalent to ∼1.5 times the thickness of a protein shell. We propose a scenario in which VLP generation may take place through monomer exchange between aggregated particles with defect-ridden or incomplete shells, leading to the formation of stable icosahedral shells, which eventually bud off the aggregate. Together with results from previous works, the findings highlight the astonishing versatility of plant virus capsid protein assembly. This previously unknown mechanism for VLP formation has features that may have relevance for the crowded environment characterizing virus factories in the cell.
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影响因子：
10.8
作者：
Chen, C;Daniel, MC;Dragnea, B
通讯作者：
Dragnea, B
DOI：
10.1021/jp802498z
发表时间：
2008-10-02
期刊：
The journal of physical chemistry. A
影响因子：
--
作者：
Chen C;Kao CC;Dragnea B
通讯作者：
Dragnea B
影响因子：
--
作者：
Aniagyei, Stella E.;DuFort, Christopher;Dragnea, Bogdan
通讯作者：
Dragnea, Bogdan
影响因子：
3
作者：
Mastronarde, DN
通讯作者：
Mastronarde, DN
影响因子：
15
作者：
Kler, Stanislav;Asor, Roi;Li, Chenglei;Ginsburg, Avi;Harries, Daniel;Oppenheim, Ariella;Zlotnick, Adam;Raviv, Uri
通讯作者：
Raviv, Uri