Ultrasonic Nanobubbles Carrying Anti-PSMA Nanobody: Construction and Application in Prostate Cancer-Targeted Imaging.
Ultrasonic Nanobubbles Carrying Anti-PSMA Nanobody: Construction and Application in Prostate Cancer-Targeted Imaging.
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携带抗PSMA纳米病的超声纳米泡：以癌症为目标的成像中的结构和应用。
DOI：
10.1371/journal.pone.0127419
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Fang K
中科院分区：
文献类型：
--
作者：
Fan X;Wang L;Guo Y;Tu Z;Li L;Tong H;Xu Y;Li R;Fang K
为了促进使用靶向分子的前列腺癌成像，我们构建了与特异性抗PSMA（前列腺特异性膜抗原）纳米抗体偶联的超声纳米泡，并评估了它们的体外结合能力和体内成像功效。通过生物素-链霉亲和素系统构建的“靶向”纳米泡具有487.60 ± 33.55 nm的平均直径，并且如通过免疫荧光所证明的携带抗PSMA纳米抗体。显微镜检查显示纳米气泡在体外与PSMA阳性细胞的靶向结合。此外，对三种动物异种移植物（LNCaP、C4-2和MKN 45）的超声成像进行了纳米气泡成像的超声指标（包括到达时间、峰值时间、峰值强度和增强持续时间）评价，结果显示靶向纳米气泡的这四项指标与空白纳米气泡相比具有显著性差异。因此，本研究不仅为靶向超声治疗前列腺癌提供了一种新的途径，也为构建小尺寸、高效率的靶向超声纳米泡提供了基础和方法。
To facilitate prostate cancer imaging using targeted molecules, we constructed ultrasonic nanobubbles coupled with specific anti-PSMA (prostate specific membrane antigen) nanobodies, and evaluated their in vitro binding capacity and in vivo imaging efficacy. The “targeted” nanobubbles, which were constructed via a biotin-streptavidin system, had an average diameter of 487.60 ± 33.55 nm and carried the anti-PSMA nanobody as demonstrated by immunofluorescence. Microscopy revealed targeted binding of nanobubbles in vitro to PSMA-positive cells. Additionally, ultrasonography indicators of nanobubble imaging (including arrival time, peak time, peak intensity and enhanced duration) were evaluated for the ultrasound imaging in three kinds of animal xenografts (LNCaP, C4-2 and MKN45), and showed that these four indicators of targeted nanobubbles exhibited significant differences from blank nanobubbles. Therefore, this study not only presents a novel approach to target prostate cancer ultrasonography, but also provides the basis and methods for constructing small-sized and high-efficient targeted ultrasound nanobubbles.
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