FUCCI sensors: powerful new tools for analysis of cell proliferation.
FUCCI sensors: powerful new tools for analysis of cell proliferation.
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DOI：
10.1002/wdev.189
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发表时间：
2015-09
期刊：
影响因子：
--
通讯作者：
Edgar BA
中科院分区：
文献类型：
--
作者：
Zielke N;Edgar BA
可视化活体生物体内单个细胞的细胞周期行为可以促进对发育过程的理解，例如模式形成、形态发生、细胞分化、生长、细胞迁移和细胞死亡。荧光泛素细胞周期指示剂 (FUCCI) 技术为实现这一目标提供了一种准确、通用且普遍适用的方法。近年来，FUCCI系统已适应多种模型系统，包括苍蝇、鱼、小鼠和植物，使该技术可供广大研究人员研究各种生物问题。此外，已经产生了多种源自不同细胞类型的表达 FUCCI 的细胞系，因此能够设计结合体内实验和基于细胞的方法（例如高内涵筛选）的高级研究。尽管推出时间不长，但 FUCCI 技术已经为细胞如何建立静止以及 G1 期长度如何影响多能性和干细胞分化之间的平衡提供了基本见解。使用 FUCCI 技术的进一步发现肯定会到来。 WIRE 开发生物学 2015 年，4：469–487。 doi: 10.1002/wdev.189 成体干细胞、组织更新和再生 > 方法和原理技术 > 生成嵌合体和谱系分析技术 > 细胞、组织和动物表型分析 本文分类如下：
Visualizing the cell cycle behavior of individual cells within living organisms can facilitate the understanding of developmental processes such as pattern formation, morphogenesis, cell differentiation, growth, cell migration, and cell death. Fluorescence Ubiquitin Cell Cycle Indicator (FUCCI) technology offers an accurate, versatile, and universally applicable means of achieving this end. In recent years, the FUCCI system has been adapted to several model systems including flies, fish, mice, and plants, making this technology available to a wide range of researchers for studies of diverse biological problems. Moreover, a broad range of FUCCI‐expressing cell lines originating from diverse cell types have been generated, hence enabling the design of advanced studies that combine in vivo experiments and cell‐based methods such as high‐content screening. Although only a short time has passed since its introduction, the FUCCI technology has already provided fundamental insight into how cells establish quiescence and how G1 phase length impacts the balance between pluripotency and stem cell differentiation. Further discoveries using the FUCCI technology are sure to come. WIREs Dev Biol 2015, 4:469–487. doi: 10.1002/wdev.189 Adult Stem Cells, Tissue Renewal, and Regeneration > Methods and Principles Technologies > Generating Chimeras and Lineage Analysis Technologies > Analysis of Cell, Tissue, and Animal Phenotypes This article is categorized under:
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影响因子：
64.5
作者：
EDGAR, BA;SCHUBIGER, G
通讯作者：
SCHUBIGER, G
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作者：
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作者：
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