GmBTB/POZ promotes the ubiquitination and degradation of LHP1 to regulate the response of soybean to Phytophthora sojae.
GmBTB/POZ promotes the ubiquitination and degradation of LHP1 to regulate the response of soybean to Phytophthora sojae.
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DOI：
10.1038/s42003-021-01907-7
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发表时间：
2021-03-19
影响因子：
5.9
通讯作者：
Xu P
中科院分区：
文献类型：
--
作者：
Zhang C;Cheng Q;Wang H;Gao H;Fang X;Chen X;Zhao M;Wei W;Song B;Liu S;Wu J;Zhang S;Xu P
大豆疫霉(Phytophthora Sojae)是引起大豆根茎腐烂的一种病原菌。Merr.)。我们先前证明，GmBTB/POZ是一种含有BTB/POZ结构域的核蛋白，通过依赖于水杨酸(SA)的过程增强大豆对大豆疫霉的抗性。在这里，我们证明了GmBTB/POZ在体内外与大豆样异染色质蛋白1(GmLHP1)直接结合，并促进其泛素化和降解。转基因株系的过表达和RNA干扰分析表明，GmLHP1通过降低SA水平和抑制GmPR1的表达，负向调控大豆对大豆疫霉病菌的反应。WRKY转录因子基因GmWRKY40是SA信号通路中SA诱导的基因，它被GmLHP1靶向，至少通过两种机制(直接与其启动子结合和阻碍SA积累)抑制其表达。此外，GmLHP1的核定位对于GmLHP1介导的免疫、SA水平的负调节和对GmWRKY40表达的抑制是必需的。最后，GmBTB/POZ在GmLHP1-OE毛状根中释放了GmLHP1调节的GmWRKY40抑制，并增加了对大豆疫霉的抗性。这些发现揭示了GmBTB/POZ-GmLHP1调控大豆对大豆疫霉病菌抗性的机制，可能是通过调控下游靶基因GmWRKY40的表达。Zhang等人描述了GmBTB/POZ增强大豆对大豆疫霉病菌抗性的机制。它们表明GmBTB/POZ直接与异染色质蛋白GmLHP1结合，并介导其降解来调节水杨酸信号通路，为防御一种常见的大豆病害提供了见解。
Phytophthora sojae is a pathogen that causes stem and root rot in soybean (Glycine max [L.] Merr.). We previously demonstrated that GmBTB/POZ, a BTB/POZ domain-containing nuclear protein, enhances resistance to P. sojae in soybean, via a process that depends on salicylic acid (SA). Here, we demonstrate that GmBTB/POZ associates directly with soybean LIKE HETEROCHROMATIN PROTEIN1 (GmLHP1) in vitro and in vivo and promotes its ubiquitination and degradation. Both overexpression and RNA interference analysis of transgenic lines demonstrate that GmLHP1 negatively regulates the response of soybean to P. sojae by reducing SA levels and repressing GmPR1 expression. The WRKY transcription factor gene, GmWRKY40, a SA-induced gene in the SA signaling pathway, is targeted by GmLHP1, which represses its expression via at least two mechanisms (directly binding to its promoter and impairing SA accumulation). Furthermore, the nuclear localization of GmLHP1 is required for the GmLHP1-mediated negative regulation of immunity, SA levels and the suppression of GmWRKY40 expression. Finally, GmBTB/POZ releases GmLHP1-regulated GmWRKY40 suppression and increases resistance to P. sojae in GmLHP1-OE hairy roots. These findings uncover a regulatory mechanism by which GmBTB/POZ-GmLHP1 modulates resistance to P. sojae in soybean, likely by regulating the expression of downstream target gene GmWRKY40. Zhang et al characterise the mechanism by which GmBTB/POZ enhances resistance to P. sojae in soybean. They show that GmBTB/POZ directly associates with the heterochromatin protein GmLHP1 and mediates its degradation to regulate the salicylic acid signaling pathway, providing insights into the defence against a common soybean disease.
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影响因子：
3.7
作者：
Dhakal R;Park E;Lee SW;Baek KH
通讯作者：
Baek KH
DOI：
10.1111/j.1365-313x.2009.03839.x
发表时间：
2009-06
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Cui H;Benfey PN
通讯作者：
Benfey PN
影响因子：
30.3
作者：
Chandran, Divya;Rickert, Joshua;Wildermuth, Mary C.
通讯作者：
Wildermuth, Mary C.
影响因子：
5.1
作者：
Chen, CH;Chen, ZX
通讯作者：
Chen, ZX
影响因子：
30.5
作者：
Ausubel, FM
通讯作者：
Ausubel, FM