Spike timing-dependent selective strengthening of single climbing fibre inputs to Purkinje cells during cerebellar development.
Spike timing-dependent selective strengthening of single climbing fibre inputs to Purkinje cells during cerebellar development.
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DOI：
10.1038/ncomms3732
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发表时间：
2013
影响因子：
16.6
通讯作者：
Kano M
中科院分区：
文献类型：
--
作者：
Kawamura Y;Nakayama H;Hashimoto K;Sakimura K;Kitamura K;Kano M
在发育中的大脑中形成功能性神经回路涉及早期形成的冗余突触的活动依赖性细化。在发育中的小脑中，爬行纤维（CF）和浦肯野细胞（PC）之间的一对一连接是通过选择性地加强单个CF，然后消除多余的CF来建立的。在这里，我们调查CF介导的反应在PC的发展变化，通过使用在体内全细胞记录和双光子Ca 2+成像。我们发现，每个新生儿PC接收时间群集的输入从多个CF和时间整合这些输入需要诱导突发尖峰和Ca 2+上升PC。重要的是，一个单一的CF输入最接近PC的尖峰输出选择性地加强在出生后的发展。在PC选择性P/Q型电压依赖性Ca 2+通道基因敲除小鼠中，这种尖峰时间依赖性选择性增强不太突出。因此，尖峰时间和Ca 2+依赖的可塑性似乎是选择一个单一的“赢家”CF和建立成熟的CF-PC连接的基础。 小脑的发育涉及到攀缘纤维和浦肯野细胞之间突触联系的活动依赖性加强。Kawamura等人表明，在特定接触加强之前，时间上聚集的多个攀爬纤维输入有助于未成熟浦肯野细胞的特征性爆发尖峰。
Shaping functional neural circuits in developing brain involves activity-dependent refinement of early-formed redundant synapses. In the developing cerebellum, a one-to-one connection between a climbing fibre (CF) and a Purkinje cell (PC) is established by selective strengthening of a single CF followed by elimination of surplus CFs. Here we investigate developmental changes in CF-mediated responses in PCs by using in vivo whole-cell recordings and two-photon Ca2+ imaging. We show that each neonatal PC receives temporally clustered inputs from multiple CFs and temporal integration of these inputs is required to induce burst spiking and Ca2+ rise in PCs. Importantly, a single CF input closest to PC’s spike output is selectively strengthened during postnatal development. This spike timing-dependent selective strengthening is much less prominent in PC-selective P/Q-type voltage-dependent Ca2+ channel knockout mice. Thus, spike timing- and Ca2+-dependent plasticity appears to underlie the selection of a single ‘winner’ CF and the establishment of mature CF–PC connections. Cerebellar development involves activity-dependent strengthening of synaptic contacts between climbing fibres and Purkinje cells. Kawamura et al. show that temporally clustered multiple climbing fibre inputs contribute to characteristic burst spiking in immature Purkinje cells before specific contacts are strengthened.
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影响因子：
3.5
作者：
Broser, PJ;Schulte, R;Wittum, G
通讯作者：
Wittum, G
影响因子：
2.5
作者：
Devor, A;Yarom, Y
通讯作者：
Yarom, Y
影响因子：
9.8
作者：
Kalinovsky A;Boukhtouche F;Blazeski R;Bornmann C;Suzuki N;Mason CA;Scheiffele P
通讯作者：
Scheiffele P
影响因子：
3.3
作者：
Andjus, PR;Zhu, L;Strata, P
通讯作者：
Strata, P
影响因子：
5.3
作者：
Blenkinsop, TA;Lang, EJ
通讯作者：
Lang, EJ