Transcriptional Regulation of Two Conceptus Interferon Tau Genes Expressed in Japanese Black Cattle during Peri-Implantation Period
Transcriptional Regulation of Two Conceptus Interferon Tau Genes Expressed in Japanese Black Cattle during Peri-Implantation Period
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日本黑牛围着床期表达的两个受精干扰素 Tau 基因的转录调控
作者：
T. Sakurai;S. Nakagawa;Min‐su Kim;H. Bai;Rulan Bai;Junyou Li;K. Min;A. Ideta;Y. Aoyagi;K. Imakawa
干扰素tau(IFNT)是由单核滋养外胚层产生的，是反刍动物母体识别怀孕的信号。然而，它在体内的表达及其转录调控还没有得到很好的描述。本研究的目的是确定在牛子宫中表达的胚胎IFNT基因亚型，并鉴定表达不同的IFNT基因启动子序列的差异。对17天、20天和22天(发情期第0天)的牛胚胎进行RNASeq数据分析，检测到两个 = 转录本IFNT1和IFNTc1的表达，它们确实被归类为IFNT基因分支。RNA-seq和定量RT-PCR分析还显示，两种IFNT基因的表达水平在第17天达到最高，然后在第20天和第22天下降。牛耳源性成纤维细胞是本实验室常用的牛IFNT基因转录研究的模型系统，它与携带IFNT1或IFNTc1基因上游(637~+51位)区域的荧光素酶报告基因构建体和不同转录因子表达载体包括CDX2、AP-1(Jun)和−共转染。CDX2单独或与其他转录因子一起显著提高了荧光素酶的活性。然后，IFNT1和IFNTc1基因座的上游区域在潜在的CDX、AP-1和ETS结合位点上连续缺失或点突变。与野生型构建物相比，CDX2或ETS2结合位点的缺失或突变同样降低了IFNT1或IFNTc1启动子构建体的荧光素酶活性。然而，随着AP-1位点的突变，IFNT1-和IFNTc1-Reports的行为不同。这些结果表明，两种形式的牛胚胎IFNT基因在子宫内表达，它们的转录调控不同。
Interferon tau (IFNT), produced by the mononuclear trophectoderm, signals the process of maternal recognition of pregnancy in ruminants. However, its expression in vivo and its transcriptional regulation are not yet well characterized. Objectives of this study were to determine conceptus IFNT gene isoforms expressed in the bovine uterus and to identify differences in promoter sequences of IFNT genes that differ in their expression. RNA-seq data analysis of bovine conceptuses on days 17, 20, and 22 (day 0 = day of estrus) detected the expression of two IFNT transcripts, IFNT1 and IFNTc1, which were indeed classified into the IFNT gene clade. RNA-seq and quantitative RT-PCR analyses also revealed that the expression levels of both IFNT mRNAs were highest on day 17, and then decreased on days 20 and 22. Bovine ear-derived fibroblast (EF) cells, a model system commonly used for bovine IFNT gene transcription study in this laboratory, were cotransfected with luciferase reporter constructs carrying upstream (positions −637 to +51) regions of IFNT1 or IFNTc1 gene and various transcription factor expression plasmids including CDX2, AP-1 (Jun) and ETS2. CDX2, either alone or with the other transcription factors, markedly increased luciferase activity. The upstream regions of IFNT1 and IFNTc1 loci were then serially deleted or point-mutated at potential CDX-, AP-1-, and ETS-binding sites. Compared to the wild-type constructs, deletion or mutation at CDX2 or ETS2 binding sites similarly reduced the luciferase activities of IFNT1- or IFNTc1-promoter constructs. However, with the AP-1 site mutated construct, IFNT1- and IFNTc1-reporters behaved differently. These results suggest that two forms of bovine conceptus IFNT genes are expressed in utero and their transcriptional regulations differ.
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根据性别和受孕年龄的牛干扰素-tau 变体的表达。
影响因子：
2.8
作者：
Walker,AngelaM;Kimura,Koji;Roberts,RMichael
通讯作者：
Roberts,RMichael
DOI：
--
发表时间：
2000
期刊：
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research
影响因子：
--
作者：
A. P. Alexenko;A. Ealy;J. Bixby;R. Roberts
通讯作者：
A. P. Alexenko;A. Ealy;J. Bixby;R. Roberts
绵羊、山羊和麝牛滋养层干扰素基因以及相关基因在哺乳动物中的分布。
DOI：
10.1089/jir.1992.12.1
发表时间：
1992
期刊：
Journal of interferon research
影响因子：
--
作者：
Leaman,DW;Roberts,RM
通讯作者：
Roberts,RM
不同的绵羊干扰素tau基因的表达并不相同，其蛋白质产物表现出不同的活性。
影响因子：
3.6
作者：
Ealy,AD;Green,JA;Alexenko,AP;Keisler,DH;Roberts,RM
通讯作者：
Roberts,RM
影响因子：
4.8
作者：
Ealy, AD;Larson, SF;Roberts, RM
通讯作者：
Roberts, RM