Matrix Stiffness Modulates Mesenchymal Stem Cell Sensitivity to Geometric Asymmetry Signals.
Matrix Stiffness Modulates Mesenchymal Stem Cell Sensitivity to Geometric Asymmetry Signals.
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DOI：
10.1007/s10439-018-2008-8
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发表时间：
2018-06
影响因子：
3.8
通讯作者：
Jabbarzadeh E
中科院分区：
文献类型：
--
作者：
Piroli ME;Jabbarzadeh E
人类干细胞具有治疗各种疾病的巨大潜力。然而，由于对它们对环境刺激作出反应的机制的了解有限，它们作为一种疗法的使用受到阻碍。努力了解细胞外生物物理线索已经证明了几何和机械信号在决定干细胞命运的关键作用。本研究的目的是探讨干细胞谱系特化中细胞极性和基质硬度之间的相互作用。我们假设将细胞限制在不对称的细胞外基质（ECM）岛上将在单细胞水平上赋予极性，并与机械信号相互作用以定义干细胞的谱系。为了测试这些假设，我们采用微接触印刷来创建具有软硬表面刚度的图案化对称和不对称水凝胶岛。人类间充质干细胞（hMSCs）被限制在这些岛屿在单细胞水平，并给予分化能力沿着脂肪或成骨路线。我们的研究结果表明，细胞极性定义的谱系规格的hMSCs只有在岛屿与低刚度。从这项研究中获得的见解为设计干细胞培养物以增强组织工程和再生医学策略提供了合理的基础。
Human stem cells hold significant potential for the treatment of various diseases. However, their use as a therapy is hampered because of limited understanding of the mechanisms by which they respond to environmental stimuli. Efforts to understand extracellular biophysical cues have demonstrated the critical roles of geometrical and mechanical signals in determining the fate of stem cells. The goal of this study was to explore the interplay between cell polarity and matrix stiffness in stem cell lineage specification. We hypothesize that confining cells to asymmetric extracellular matrix (ECM) islands will impart polarity at a single cell-level and will interact with mechanical signals to define the lineage of stem cells. To test these hypotheses, we employed microcontact printing to create patterned symmetric and asymmetric hydrogel islands of soft and hard surface stiffness. Human mesenchymal stem cells (hMSCs) were confined to these islands at the single-cell level and given the ability to differentiate along adipogenic or osteogenic routes. Our results demonstrated that cell polarity defines the lineage specification of hMSCs only on islands with low stiffness. Insight gained from this study provides a rational basis for designing stem cell cultures to enhance tissue engineering and regenerative medicine strategies.
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影响因子：
5.6
作者：
MOONEY, D;HANSEN, L;INGBER, D
通讯作者：
INGBER, D
DOI：
10.1073/pnas.0903269107
发表时间：
2010-03-16
影响因子：
11.1
作者：
Kilian, Kristopher A.;Bugarija, Branimir;Mrksich, Milan
通讯作者：
Mrksich, Milan
影响因子：
5.2
作者：
Krtolica, Ana;Genbacev, Olga;Fisher, Susan J.
通讯作者：
Fisher, Susan J.
影响因子：
64.8
作者：
Morrison, Sean J.;Kimble, Judith
通讯作者：
Kimble, Judith
影响因子：
19
作者：
Harris, Greg M.;Piroli, Maria E.;Jabbarzadeh, Ehsan
通讯作者：
Jabbarzadeh, Ehsan