Cytokinesis-required Cdc14 is a signaling hub of asexual development and multi-stress tolerance in Beauveria bassiana.
Cytokinesis-required Cdc14 is a signaling hub of asexual development and multi-stress tolerance in Beauveria bassiana.
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细胞分裂所需的 Cdc14 是白僵菌无性发育和多重胁迫耐受性的信号中枢
DOI：
10.1038/srep03086
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发表时间：
2013-10-30
影响因子：
4.6
通讯作者：
Feng, Ming-Guang
中科院分区：
文献类型：
--
作者：
Wang, Jie;Liu, Jing;Hu, Yue;Ying, Sheng-Hua;Feng, Ming-Guang
双重特异性，无旁系同源性CDC14磷酸酶位于Bassiana的核（丝状昆虫病原体）中，并在功能上表征了CDC14的灭活。在正常条件下的生长和≥96％的分生孢子产量。此外，ΔCDC14对氧化物，渗透和细胞壁和有丝分裂的压力变得过敏，并损失了分生孢子耐热性，UV-B耐药性和病毒的41-70％，通过各种信号传导因素和应激效应的转录下调而实现HOG1和SLT2在高渗透性甘油和细胞壁完整性途径中的抑郁磷酸化信号通过营救CDC14来恢复。我们的发现表明，对真菌细胞力的CDC14不仅是无性发育，而且是多应力反应和病毒的信号枢纽。
A dual-specificity, paralogue-free Cdc14 phosphatase was located in the nuclei of Beauveria bassiana (filamentous entomopathogen) and functionally characterized. Inactivation of cdc14 caused defective cytokinesis due to multinucleate cells formed in Δcdc14 and 89% decrease of blastospore production, followed by slower growth and a loss of ≥ 96% conidial yield under normal conditions. These defects coincided well with drastic down-regulation of 25 genes required for mitosis and conidiation. Moreover, Δcdc14 became hypersensitive to oxidative, osmotic, and cell wall and mitosis perturbing stresses, and lost 41−70% of conidial thermotolerance, UV-B resistance and virulence, accompanied with transcriptional down-regualtion of various signaling factors and stress-responsive effectors and depressed phosphorylation signals of Hog1 and Slt2 in high-osmolarity glycerol and cell-wall integrity pathways. All changes were well restored by rescuing cdc14. Our findings indicate that Cdc14 vital for the fungal cytokinesis acts as a signaling hub in regulating not only asexual development but multi-stress responses and virulence.
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DOI：
10.1083/jcb.200202054
发表时间：
2002-09-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gruneberg U;Glotzer M;Gartner A;Nigg EA
通讯作者：
Nigg EA
基于检测抗原85复合物的间接ELISA方案，印度人群中结核病的诊断：一项前瞻性队列研究。
影响因子：
3.7
作者：
Kashyap, Rajpal S.;Rajan, Anju N.;Ramteke, Sonali S.;Agrawal, Vijay S.;Kelkar, Sanjivani S.;Purohit, Hemant J.;Taori, Girdhar M.;Daginawala, Hatim F.
通讯作者：
Daginawala, Hatim F.
影响因子：
3.3
作者：
FANKHAUSER, C;SIMANIS, V
通讯作者：
SIMANIS, V
影响因子：
4
作者：
Mocciaro, Annamaria;Schiebel, Elmar
通讯作者：
Schiebel, Elmar
影响因子：
64.5
作者：
Colman-Lerner, A;Chin, TE;Brent, R
通讯作者：
Brent, R