Highly parallel fabrication of nanopatterned surfaces with nanoscale orthogonal biofunctionalization imprint lithography

Highly parallel fabrication of nanopatterned surfaces with nanoscale orthogonal biofunctionalization imprint lithography
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利用纳米级正交生物功能化压印光刻高度并行制造纳米图案表面

DOI:
10.1088/0957-4484/18/13/135101
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发表时间:
2007
期刊:
影响因子:
3.5
通讯作者:
W. Frey
W. Frey
中科院分区:
材料科学3区
文献类型:
--
作者:
Harold E Gaubert;W. Frey

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生物实验和生物技术应用需要大面积的具有特定化学功能的纳米图案。我们提出了纳米级正交生物功能化压印光刻(NOBIL),这是一种基于步进闪光压印光刻(SFIL)的并行自上而下压印和剥离技术,能够创建具有两种生化功能的厘米级纳米图案区域。光刻胶前体与模板一起聚合,然后蚀刻薄光刻胶层以形成用于剥离的底切。然后,二氧化硅背景上的金纳米区域通过自组装进行独立功能化,将纳米图案转化为细胞粘附/细胞排斥功能图案。我们通过在聚乙二醇 (PEG) 背景下创建小至 60 nm 图案大小的纤连蛋白区域来演示该技术,并显示细胞稳定接种在尺寸和间距受控的 1 mm2 区域阵列上的初步结果。
Large areas of nanopatterns of specific chemical functionality are needed for biological experiments and biotechnological applications. We present nanoscale orthogonal biofunctionalization imprint lithography (NOBIL), a parallel top-down imprinting and lift-off technique based on step-and-flash imprint lithography (SFIL) that is able to create centimetre-scale areas of nanopatterns of two biochemical functionalities. A photoresist precursor is polymerized with a template in place, and the thin resist layer is etched to create an undercut for lift-off. Gold nano-areas on a silicon dioxide background are then independently functionalized using self-assembly that translates the nanopattern into a cell-adhesive/cell-rejective functionality pattern. We demonstrate the technique by creating fibronectin areas down to a pattern size of 60 nm against a polyethylene glycol (PEG) background, and show initial results of cells stably seeded over an array of 1 mm2 areas of controlled size and pitch.
DOI: 10.1126/science.276.5317.1425
发表时间: 1997-05-30
期刊: SCIENCE
影响因子: 56.9
作者:
Chen, CS;Mrksich, M;Ingber, DE
通讯作者: Ingber, DE
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发表时间: 2002
影响因子: 4
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