Stretch-activated force shedding, force recovery, and cytoskeletal remodeling in contractile fibroblasts.
Stretch-activated force shedding, force recovery, and cytoskeletal remodeling in contractile fibroblasts.
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DOI：
10.1016/j.jbiomech.2008.07.033
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发表时间：
2008-10-20
影响因子：
2.4
通讯作者：
Genin, Guy M.
中科院分区：
文献类型：
--
作者：
Nekouzadeh, Ali;Pryse, Kenneth M.;Elson, Elliot L.;Genin, Guy M.
收缩成纤维细胞内的应力纤维网络在结构上加强了组织，并为愈合伤口中的组织提供了张力或“张力”。以前已经观察到应力纤维在机械力的作用下聚合。我们观察到，当充分拉伸时，收缩成纤维细胞通过解聚肌动蛋白细丝来减少它们对环境施加的机械牵引力，然后通过分阶段的钙离子依赖过程恢复组织张力并重建肌动蛋白应激纤维。这些由钙离子调节的阶段性收缩包括一个在伸展后不到一分钟结束的快速阶段，一个静止的平台期，以及一个需要几分钟才能完成的最后的渐进阶段。肌动蛋白细胞骨架的重建程度与恢复期的主动收缩力成正比。这种互补的作用展示了一种程序化的调节机制，通过精心设计的主动机械和生化愈合反应来保护细胞免受过度拉伸。
The stress fiber network within contractile fibroblasts structurally reinforces and provides tension, or “tone”, to tissues such as those found in healing wounds. Stress fibers have previously been observed to polymerize in response to mechanical forces. We observed that, when stretched sufficiently, contractile fibroblasts diminished the mechanical tractions they exert on their environment through depolymerization of actin filaments then restored tissue tension and rebuilt actin stress fibers through staged Ca++–dependent processes. These staged Ca++–modulated contractions consisted of a rapid phase that ended less than a minute after stretching, a plateau of inactivity, and a final gradual phase that required several minutes to complete. Active contractile forces during recovery scaled with the degree of rebuilding of the actin cytoskeleton. This complementary action demonstrates a programmed regulatory mechanism that protects cells from excessive stretch through choreographed active mechanical and biochemical healing responses.
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影响因子：
64.5
作者：
KREIS, TE;BIRCHMEIER, W
通讯作者：
BIRCHMEIER, W
DOI：
10.1073/pnas.0605837103
发表时间：
2006-09-19
影响因子：
11.1
作者：
Deshpande, Vikram S.;McMeeking, Robert M.;Evans, Anthony G.
通讯作者：
Evans, Anthony G.
影响因子：
5.5
作者：
Mott, Rosalind E.;Helmke, Brian P.
通讯作者：
Helmke, Brian P.
影响因子：
2.9
作者：
Prajapati, RT;Chavally-Mis, B;Brown, RA
通讯作者：
Brown, RA
DOI：
10.1083/jcb.117.1.73
发表时间：
1992-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kolodney MS;Wysolmerski RB
通讯作者：
Wysolmerski RB