18 Additional Amino Acids of the Signal Peptide of the Bombyx mori Nucleopolyhedrovirus GP64 Activates Immunoglobulin Binding Protein (BiP) Expression by RNA-seq Analysis
18 Additional Amino Acids of the Signal Peptide of the Bombyx mori Nucleopolyhedrovirus GP64 Activates Immunoglobulin Binding Protein (BiP) Expression by RNA-seq Analysis
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通过 RNA-seq 分析发现家蚕核多角体病毒 GP64 信号肽的 18 个附加氨基酸激活免疫球蛋白结合蛋白 (BiP) 表达
DOI：
10.1007/s00284-020-02309-4
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发表时间：
2021-01
影响因子：
2.6
通讯作者：
Bifang Hao
中科院分区：
文献类型：
--
作者：
Na Liu;Jinshan Huang;Lin Liu;Frank Boadi;Yanhui Song;Zhongjian Guo;Xingjia Shen;Bifang Hao
GP64 是 I 类杆状病毒的关键膜融合蛋白，虽然家蚕核多角体病毒 (BmNPV) GP64 含有比苜蓿银纹夜蛾多核多角体病毒 (AcMNPV) 更长的信号肽 n 区（18 个氨基酸），但 n 区的功能尚未确定。在本研究中，我们首先证明了BmN细胞中膜蛋白定位需要n区，然后对不同信号肽区域引导的蛋白进行转录组测序，并通过定量PCR和荧光素酶测定对结果进行分析和验证。结果表明，信号肽与对照不同区域共鉴定出1049个差异表达基因（DEG）。随着n区的增加，蛋白输出通路显着上调，Wnt-1信号通路下调，BiP被GP64全长信号肽显着激活。此外，对 BiP 的 RNA 干扰有效地增加了荧光素酶的分泌。这些结果表明 GP64 n 区在蛋白质表达和调节中发挥关键作用。
GP64 is the key membrane fusion protein of Group I baculovirus, and while the Bombyx mori nucleopolyhedrovirus (BmNPV) GP64 contains a longer n-region (18 amino acid) of the signal peptide than does the Autographa californica multiple nucleopolyhedrovirus (AcMNPV), the function of the n-region has not been determined. In this study, we first showed that n-region is required for membrane protein localization in BmN cells, then the transcriptome sequencing was conducted on proteins guided by different signal peptide regions, and the results were analyzed and validated by quantitative PCR and luciferase assays. The results indicated that 1049 differentially expressed genes (DEGs) were identified among the different region of signal peptides and the control. With the n-region, the protein export pathway was upregulated significantly, the Wnt-1 signaling pathway was downregulated, and BiP was significantly activated by the GP64 full-length signal peptide. Furthermore, RNA interference on BiP efficiently increased luciferase secretion. These results indicate that the GP64 n-region plays a key role in protein expression and regulation.
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影响因子：
5.4
作者：
Harmon B;Bird SW;Schudel BR;Hatch AV;Rasley A;Negrete OA
通讯作者：
Negrete OA
影响因子：
3.5
作者：
Kamita, SG;Maeda, S
通讯作者：
Maeda, S
影响因子：
3.8
作者：
Gomi, S;Majima, K;Maeda, S
通讯作者：
Maeda, S
影响因子：
5
作者：
Liu, Hanyang;Wu, Rui;Wen, Xintian
通讯作者：
Wen, Xintian
DOI：
10.1046/j.1432-1033.2002.03135.x
发表时间：
2002-09
期刊：
European journal of biochemistry
影响因子：
--
作者：
A. Spenger;R. Grabherr;Lars Töllner;H. Katinger;W. Ernst
通讯作者：
A. Spenger;R. Grabherr;Lars Töllner;H. Katinger;W. Ernst