Gene expression profile analysis of tobacco leaf trichomes.
Gene expression profile analysis of tobacco leaf trichomes.
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DOI：
10.1186/1471-2229-11-76
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发表时间：
2011-05-08
影响因子：
5.3
通讯作者：
Wang XJ
中科院分区：
文献类型：
--
作者：
Cui H;Zhang ST;Yang HJ;Ji H;Wang XJ
烟草的叶毛以其尺寸大、密度高和优异的分泌能力而著称。它们有助于植物针对生物和非生物胁迫的防御反应，还影响叶香和烟味。然而，有关这种非模式植物物种毛状体的基因组信息有限。我们使用两种方法表征了烟草叶毛状体基因表达。首先，对毛状体 cDNA 文库进行了随机测序，获得了 2831 个独特基因。最丰富的转录物是核酮糖二磷酸羧化酶（RuBisCO）。在相关序列中，大多数编码的酶参与初级代谢。还鉴定了次级代谢相关基因，例如类异戊二烯和类黄酮生物合成相关基因。在第二种方法中，使用从这 2831 个克隆制备的 cDNA 微阵列来比较毛状体和叶子中的基因表达水平。毛状体和叶减毛状体之间有438个差异表达基因。其中，烟草毛状体中高表达的207个基因在第二代谢过程、防御反应和代谢调节类别中富集。通过半定量RT-PCR分析证实了所选unigenes的表达，其中一些在毛状体中特异性表达。烟草叶毛状体的表达特征表明它们的代谢活性和在抗逆性中的潜在重要性。主要在毛状体中表达的序列将促进植物毛状体的基因挖掘和代谢控制。
Leaf trichomes of Nicotiana tabacum are distinguished by their large size, high density, and superior secretion ability. They contribute to plant defense response against biotic and abiotic stress, and also influence leaf aroma and smoke flavor. However, there is limited genomic information about trichomes of this non-model plant species. We have characterized Nicotiana tabacum leaf trichome gene expression using two approaches. In the first, a trichome cDNA library was randomly sequenced, and 2831 unique genes were obtained. The most highly abundant transcript was ribulose bisphosphate carboxylase (RuBisCO). Among the related sequences, most encoded enzymes involved in primary metabolism. Secondary metabolism related genes, such as isoprenoid and flavonoid biosynthesis-related, were also identified. In the second approach, a cDNA microarray prepared from these 2831 clones was used to compare gene expression levels in trichome and leaf. There were 438 differentially expressed genes between trichome and leaves-minus-trichomes. Of these, 207 highly expressed genes in tobacco trichomes were enriched in second metabolic processes, defense responses, and the metabolism regulation categories. The expression of selected unigenes was confirmed by semi-quantitative RT-PCR analysis, some of which were specifically expressed in trichomes. The expression feature of leaf trichomes in Nicotiana tabacum indicates their metabolic activity and potential importance in stress resistance. Sequences predominantly expressed in trichomes will facilitate gene-mining and metabolism control of plant trichome.
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