Only one of the two type VI secretion systems encoded in the Salmonella enterica serotype Dublin genome is involved in colonization of the avian and murine hosts.
Only one of the two type VI secretion systems encoded in the Salmonella enterica serotype Dublin genome is involved in colonization of the avian and murine hosts.
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DOI：
10.1186/1297-9716-45-2
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发表时间：
2014-01-09
影响因子：
4.4
通讯作者：
Contreras I
中科院分区：
文献类型：
--
作者：
Pezoa D;Blondel CJ;Silva CA;Yang HJ;Andrews-Polymenis H;Santiviago CA;Contreras I
VI型分泌系统（T6 SS）是许多革兰氏阴性菌的毒力因子。沙门氏菌属具有五个遗传学上不同的T6 SS位点，其编码于沙门氏菌致病性岛（SPI）SPI-6、SPI-19、SPI-20、SPI-21和SPI-22中，其在血清型之间差异分布。SPI-6和SPI-19中编码的T6 SS分别在小鼠和鸡中促成鼠伤寒和鸡伤寒血清型的发病。都柏林沙门氏菌是一种仅限于牛的病原体，在那里它引起全身性疾病。此外，它可以定殖其他宿主，如鸡和小鼠，这些宿主可以作为这种血清型的宿主。都柏林沙门氏菌携带T6 SSSPI-6和T6 SSSPI-19的基因。本研究使用禽类和鼠类感染模型确定了T6 SSSPI-6和T6 SSSPI-19对沙门氏菌都柏林宿主定殖的贡献。竞争指数实验表明，缺乏两个T6 SSs的突变株（T6 SSSPI-6/T6 SSSPI-19）在鸡盲肠中表现出强烈的定殖缺陷，类似于对T6 SSSPI-6突变体观察到的缺陷，这表明该血清型需要功能性T6 SSSPI-6来有效地定殖禽类胃肠道。小鼠的定植也有缺陷，尽管程度低于鸡。相反，T6 SSSPI-19对于鸡或小鼠的定殖不是必需的。转移T6 SSSPI-6，而不是T6 SSSPI-19，恢复了双突变体定殖两种动物宿主的能力。我们的数据表明，沙门氏菌都柏林只需要T6 SSSPI-6的有效定植的小鼠和鸡，T6 SSSPI-6和T6 SSSPI-19不是功能冗余。
The type VI secretion system (T6SS) is a virulence factor for many Gram-negative bacteria. Salmonella genus harbors five phylogenetically distinct T6SS loci encoded in Salmonella Pathogenicity Islands (SPIs) SPI-6, SPI-19, SPI-20, SPI-21 and SPI-22, which are differentially distributed among serotypes. The T6SSs encoded in SPI-6 and SPI-19 contribute to pathogenesis of serotypes Typhimurium and Gallinarum in mice and chickens, respectively. Salmonella Dublin is a pathogen restricted to cattle where it causes a systemic disease. Also, it can colonize other hosts such as chickens and mice, which can act as reservoirs of this serotype. Salmonella Dublin harbors the genes for both T6SSSPI-6 and T6SSSPI-19. This study has determined the contribution of T6SSSPI-6 and T6SSSPI-19 to host-colonization by Salmonella Dublin using avian and murine models of infection. Competitive index experiments showed that, a mutant strain lacking both T6SSs (∆T6SSSPI-6/∆T6SSSPI-19) presents a strong colonization defect in cecum of chickens, similar to the defect observed for the ∆T6SSSPI-6 mutant, suggesting that this serotype requires a functional T6SSSPI-6 for efficient colonization of the avian gastrointestinal tract. Colonization of mice was also defective, although to a lesser extent than in chickens. In contrast, the T6SSSPI-19 was not necessary for colonization of either chickens or mice. Transfer of T6SSSPI-6, but not T6SSSPI-19, restored the ability of the double mutant to colonize both animal hosts. Our data indicate that Salmonella Dublin requires only the T6SSSPI-6 for efficient colonization of mice and chickens, and that the T6SSSPI-6 and T6SSSPI-19 are not functionally redundant.
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影响因子：
6.7
作者：
Schwarz S;West TE;Boyer F;Chiang WC;Carl MA;Hood RD;Rohmer L;Tolker-Nielsen T;Skerrett SJ;Mougous JD
通讯作者：
Mougous JD
影响因子：
4.2
作者：
Sivula CP;Bogomolnaya LM;Andrews-Polymenis HL
通讯作者：
Andrews-Polymenis HL
影响因子：
3.1
作者：
Blondel, Carlos J.;Jimenez, Juan C.;Contreras, Ines
通讯作者：
Contreras, Ines
DOI：
10.1073/pnas.1005566107
发表时间：
2010-08-31
影响因子：
11.1
作者：
Libby, Stephen J.;Brehm, Michael A.;Fang, Ferric C.
通讯作者：
Fang, Ferric C.
影响因子：
6.7
作者：
Fookes M;Schroeder GN;Langridge GC;Blondel CJ;Mammina C;Connor TR;Seth-Smith H;Vernikos GS;Robinson KS;Sanders M;Petty NK;Kingsley RA;Bäumler AJ;Nuccio SP;Contreras I;Santiviago CA;Maskell D;Barrow P;Humphrey T;Nastasi A;Roberts M;Frankel G;Parkhill J;Dougan G;Thomson NR
通讯作者：
Thomson NR