A comparison of cecal colonization of Salmonella enterica serotype Typhimurium in white leghorn chicks and Salmonella-resistant mice.
A comparison of cecal colonization of Salmonella enterica serotype Typhimurium in white leghorn chicks and Salmonella-resistant mice.
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DOI：
10.1186/1471-2180-8-182
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发表时间：
2008-10-16
期刊：
影响因子：
4.2
通讯作者：
Andrews-Polymenis HL
中科院分区：
文献类型：
--
作者：
Sivula CP;Bogomolnaya LM;Andrews-Polymenis HL
沙门氏菌病是世界范围内最重要的细菌性食源性疾病之一。人类感染的一个主要来源是食用被肠道沙门氏菌血清型鼠伤寒污染的鸡肉或鸡蛋产品，但我们对鸡肉中的定植和持久性因素的了解很少。我们比较了1周龄白色来航鸡和沙门氏菌耐药CBA/J小鼠在感染沙门氏菌血清型鼠伤寒ATCC 14028（最常研究的分离株之一）期间的肠道和全身定植。我们还研究了竞争感染过程中野生型血清型鼠伤寒ATCC 14028和一个同基因invA突变体在1周龄白色来航鸡和8周龄CBA/J小鼠盲肠中的分布。我们发现，尽管在两种感染的动物模型中鼠伤寒血清型的全身水平较低，但感染的小鼠在感染后15天开始具有显著的脾肿大。在肠道中，盲肠内容物是1周龄雏鸡和沙门氏菌抗性小鼠盲肠中鼠伤寒血清型恢复的主要部位。此外，我们表明，只有一小部分沙门氏菌在两种感染动物模型的盲肠上皮细胞内，而SPI-1是重要的成功感染的鼠模型，它是重要的与盲肠上皮1周龄小鸡。最后，我们表明，在鸡感染血清型鼠伤寒在1周龄，这种生物体的粪便脱落的水平并不反映盲肠定植的水平，因为它在小鼠模型。在我们的研究中，我们强调了鸡和鼠血清型鼠伤寒感染之间的系统和肠道定植水平的重要差异，并提供证据表明SPI-1的作用可能是不一样的，在两种动物模型的定植。
Salmonellosis is one of the most important bacterial food borne illnesses worldwide. A major source of infection for humans is consumption of chicken or egg products that have been contaminated with Salmonella enterica serotype Typhimurium, however our knowledge regarding colonization and persistence factors in the chicken is small. We compared intestinal and systemic colonization of 1-week-old White Leghorn chicks and Salmonella-resistant CBA/J mice during infection with Salmonella enterica serotype Typhimurium ATCC14028, one of the most commonly studied isolates. We also studied the distribution of wild type serotype Typhimurium ATCC14028 and an isogenic invA mutant during competitive infection in the cecum of 1-week-old White Leghorn chicks and 8-week-old CBA/J mice. We found that although the systemic levels of serotype Typhimurium in both infected animal models are low, infected mice have significant splenomegaly beginning at 15 days post infection. In the intestinal tract itself, the cecal contents are the major site for recovery of serotype Typhimurium in the cecum of 1-week-old chicks and Salmonella-resistant mice. Additionally we show that only a small minority of Salmonellae are intracellular in the cecal epithelium of both infected animal models, and while SPI-1 is important for successful infection in the murine model, it is important for association with the cecal epithelium of 1-week-old chicks. Finally, we show that in chicks infected with serotype Typhimurium at 1 week of age, the level of fecal shedding of this organism does not reflect the level of cecal colonization as it does in murine models. In our study, we highlight important differences in systemic and intestinal colonization levels between chick and murine serotype Typhimurium infections, and provide evidence that suggests that the role of SPI-1 may not be the same during colonization of both animal models.
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影响因子：
3.1
作者：
Beal, RK;Powers, C;Smith, AL
通讯作者：
Smith, AL
影响因子：
2.8
作者：
BARROW, PA;SIMPSON, JM;LOVELL, MA
通讯作者：
LOVELL, MA
影响因子：
3.6
作者：
FRANCIS, CL;STARNBACH, MN;FALKOW, S
通讯作者：
FALKOW, S
影响因子：
2.4
作者：
BARROW, PA;HUGGINS, MB;SIMPSON, JM
通讯作者：
SIMPSON, JM
影响因子：
3.1
作者：
Henderson, SC;Bouous, DI;Lee, MD
通讯作者：
Lee, MD