Microbiome Profiling of Enterotoxigenic Escherichia coli (ETEC) Carriers Highlights Signature Differences between Symptomatic and Asymptomatic Individuals.
Microbiome Profiling of Enterotoxigenic Escherichia coli (ETEC) Carriers Highlights Signature Differences between Symptomatic and Asymptomatic Individuals.
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产肠毒素大肠杆菌 (ETEC) 是低收入和中等收入国家 (LMIC) 儿童腹泻的重要原因。然而，针对儿童的大规模病原体负荷研究已在有症状的患者和对照者的肠道中发现了 ETEC。人们对影响这种平衡的因素知之甚少，但据推测肠道微生物组可能在疾病的抵抗或进展中发挥作用。在这项研究中，我们分析了孟加拉国无症状或有症状感染 ETEC 的儿童和成人的微生物组。有症状的患者具有大量与大肠杆菌和两种 ETEC 毒素相对应的测序读数，表明细菌负荷更高。他们也明显更有可能同时感染肠聚集性大肠杆菌 (EAEC)，并且其他伽马变形菌的比例也更高，包括克雷伯氏菌、沙门氏菌和嗜血杆菌。 ETEC 定植还与抗菌素耐药性 (AMR) 基因患病率增加相关，尤其是 β-内酰胺酶类基因。尽管这些变化的方向在成人和儿童中不同，但所有群体之间的分类特征在物种丰富度和组成上都存在明显不同。如前所述，大肠杆菌负荷较高的儿童的链球菌属比例也较高，而健康儿童的双歧杆菌属较多。我们的研究深入了解了地方性环境中 ETEC 感染后发生的微生物组变化，并为未来研究微生物组如何保护或使个体易受胃肠道病原体症状感染的研究提供了理论基础。
Enterotoxigenic Escherichia coli (ETEC) is an important cause of diarrhea in children in low- and middle-income countries (LMICs). However, large-scale pathogen burden studies in children have identified ETEC in the guts of both symptomatic patients and controls. The factors that influence this balance are poorly understood, but it is postulated that the gut microbiome may play a role in either resistance or progression to disease. In this study, we profiled the microbiomes of children and adults from Bangladesh who were asymptomatically or symptomatically infected with ETEC. Symptomatic patients had significantly higher numbers of sequenced reads mapping to both E. coli and two ETEC toxins, suggesting higher bacterial burden. They were also significantly more likely to be coinfected with enteroaggregative E. coli (EAEC) and had higher proportions of other Gammaproteobacteria, including Klebsiella, Salmonella, and Haemophilus. Colonization with ETEC was also associated with increased prevalence of antimicrobial resistance (AMR) genes, most notably those of the β-lactamase class. Taxonomic profiles were distinctly different between all groups in both species richness and composition, although the direction of these changes was different in adults and children. As seen previously, children with high E. coli burdens also had higher proportions of Streptococcus spp., while healthy children were more heavily colonized by Bifidobacterium spp. Our study provides insight into the microbiome changes that occur upon infection with ETEC in an endemic setting and provides rationale for future studies investigating how the microbiome may protect or predispose individuals to symptomatic infections with gastrointestinal pathogens.
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影响因子：
48
作者：
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34.3
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