Immunohistochemical Localization of HLA-DR-positive Cells in Unerupted and Erupted Normal and Carious Human Teeth
Immunohistochemical Localization of HLA-DR-positive Cells in Unerupted and Erupted Normal and Carious Human Teeth
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未萌出和萌出的正常和龋齿人类牙齿中 HLA-DR 阳性细胞的免疫组织化学定位
DOI：
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
H. Ozawa
中科院分区：
文献类型：
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作者：
N. Yoshiba;K. Yoshiba;Hiroaki Nakamura;M. Iwaku;H. Ozawa
第二类主要组织相容性复合体(MHC)抗原表达细胞通常与免疫反应的早期阶段有关。我们研究了II类表达细胞在发育中的、正常的和龋齿的人类牙齿中的分布，以阐明人类牙髓何时获得免疫防御潜力以及这种免疫防御对龋齿的反应。采用免疫组织化学方法，用人类白细胞抗原-DR单抗鉴定抗原表达细胞。在未萌出的发育牙髓中，大量的人类白细胞抗原-DR阳性细胞主要分布在成牙本质细胞层及其周围。在萌出的牙齿中，大部分HLA-DR阳性细胞位于成牙本质细胞层下方，细长的细胞质突起延伸到成牙本质细胞层中。浅表性龋损可引起相应牙髓中的HLA-DR阳性细胞聚集。在龋损较深的牙齿中，这种细胞聚集扩大到包括成牙本质细胞层。此外，牙髓-牙本质交界处可见HLA-DR阳性细胞，胞浆突起深入牙本质小管，与成牙本质细胞突起共同定位。这些结果表明：(1)人类牙髓在萌出前具有免疫防御能力；(2)在龋病感染的初期，人类牙髓中启动了由表达第II类分子的细胞介导的免疫反应；(3)随着龋病的进展，人类白细胞抗原-DR阳性细胞侵入牙本质小管。
Class II major histocompatibility complex (MHC) antigen-expressing cells are generally associated with the early phase of the immune response. We have studied the distribution of class II-expressing cells in developing, normal, and carious human teeth to clarify when human pulp acquires an immunologic defense potential and how this reacts to dental caries. Antigen-expressing cells were identified immunohistochemically by means of HLA-DR monoclonal antibody. In the pulp of unerupted developing teeth, numerous HLA-DR-positive cells were distributed mainly in and around the odontoblast layer. In erupted teeth, HLA-DR-positive cells were located, for the most part, just beneath the odontoblast layer, with slender cytoplasmic processes extending into the layer. Superficial caries lesions caused an aggregation of HLA-DR-positive cells in dental pulp corresponding to the lesion. In teeth with deeper caries lesions, this aggregation of cells expanded to include the odontoblast layer. Also noted were HLA-DR-positive cells lying along the pulp-dentin border, with cytoplasmic processes projecting deep into the dentinal tubules, where they co-localized with odontoblast processes. These findings suggest that: (1) human dental pulp is equipped with immunologic defense potential prior to eruption; (2) in the initial stage of caries infection, an immunoresponse mediated by class-II-expressing cells is initiated in human dental pulp; and (3) HLA-DR-positive cells trespass deep into dentinal tubules as the caries lesion advances.
影响因子：
4.4
作者：
Y. Nong;R. Titus;J. Ribeiro;H. Remold
通讯作者：
Y. Nong;R. Titus;J. Ribeiro;H. Remold
影响因子：
56.9
作者：
M. Lotz;J. Vaughan;D. Carson
通讯作者：
M. Lotz;J. Vaughan;D. Carson
影响因子：
4.2
作者：
BYERS, MR;TAYLOR, PE;KIMBERLY, CL
通讯作者：
KIMBERLY, CL