System-wide analysis of manganese starvation-induced metabolism in key elements of Lactobacillus plantarum
System-wide analysis of manganese starvation-induced metabolism in key elements of Lactobacillus plantarum
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锰饥饿诱导的植物乳杆菌关键元素代谢的全系统分析
DOI：
10.1039/c7ra00072c
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发表时间：
2017-02
期刊：
影响因子：
3.9
通讯作者：
Chen Wei
中科院分区：
文献类型：
--
作者：
Tong Yanjun;Zhai Qixiao;Wang Gang;Zhang Qiuxiang;Liu Xiaoming;Tian Fengwei;Zhao Jianxin;Zhang Hao;Chen Wei
为了分析植物乳杆菌对锰饥饿胁迫的响应机制，从生理、蛋白质组学和转运蛋白等方面对植物乳杆菌CCFM436的不同代谢进行了系统研究。细胞生长动力学(μ_(Max))从0.310 h−_1降至0.256 h·min~(-1)，但在缺锰条件下，细胞形态变薄。气相色谱-质谱法分析表明，膜的流动性和致密性增加，不饱和脂肪酸和环丙烷脂肪酸的比例增加。高效液相色谱分析表明，与能量代谢密切相关的细胞内天冬氨酸、谷氨酸和精氨酸含量显著增加。傅里叶变换红外光谱分析表明，饥饿对某些官能团(N-H和OC-OH)有显著影响。比较二维蛋白质组学分析鉴定出在锰饥饿条件下差异显著的73个蛋白质。这些差异表达的蛋白质涉及碳水化合物、氨基酸、转录/翻译代谢和胁迫反应，被归类为抵抗锰饥饿胁迫所需的关键成分。此外，qRT-PCR分析证明，在锰饥饿条件下，受MntR负调控的mnth1-5是潜在的锰进口体。所提出的协调机制模型为实验室菌株的细胞内代谢提供了参考和洞察。
To analyze the response mechanisms of Lactobacillus plantarum against manganese starvation stress, different metabolisms from physiology, proteomics and transporters aspects in L. plantarum CCFM 436 were systematically investigated. The kinetics of cell growth (μmax) decreased from 0.310 to 0.256 h−1, while thinner cell morphology was observed by transmission electron microscopy under Mn-starvation conditions. Gas chromatography-mass spectrometry analysis indicated that membrane mobility and compactness increased, with a higher proportion of unsaturated fatty acids and cyclopropane fatty acids. High-performance liquid chromatography analysis showed that intracellular Asp, Glu, and Arg contents, closely related to energy metabolism, were significantly increased. Fourier transform infrared spectroscopy proved that some functional groups (N–H and OC–OH) were significantly affected by Mn starvation. Comparative two-dimensional proteomic analysis identified 73 proteins that differed significantly under Mn starvation conditions. These differentially expressed proteins involved in carbohydrate, amino acid and transcription/translation metabolisms and stress response were categorized as crucial components required to resist manganese starvation stress. Moreover, qRT-PCR analysis proved that MntH 1–5, negatively regulated by MntR, acted as potential Mn importers under Mn starvation conditions. The proposed coordinated mechanism model provides a reference for, and insight into, the intracellular metabolism of LAB strains.
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影响因子：
3.9
作者：
Zhao, Jianxin;Zhang, Hao;Chen, Yong Q.;Chen, Wei
通讯作者：
Chen, Wei
影响因子：
5.3
作者：
Khadidja Senouci-Rezkallah;P. Schmitt;M. Jobin
通讯作者：
Khadidja Senouci-Rezkallah;P. Schmitt;M. Jobin
影响因子：
3.9
作者：
Y. Tong;Wang Gang;Qiuxiang Zhang;F. Tian;Xiaoming Liu;Jianxin Zhao;Hao Zhang;Wei Chen
通讯作者：
Y. Tong;Wang Gang;Qiuxiang Zhang;F. Tian;Xiaoming Liu;Jianxin Zhao;Hao Zhang;Wei Chen
DOI：
10.1016/b978-0-08-088504-9.00002-7
发表时间：
2011
期刊：
--
影响因子：
--
作者：
P. Newsholme;L. Stenson;M. Sulvucci;R. Sumayao;M. Krause
通讯作者：
P. Newsholme;L. Stenson;M. Sulvucci;R. Sumayao;M. Krause
影响因子：
2.1
作者：
Boehmer, Nico;Koenig, Saskia;Fischer, Lutz
通讯作者：
Fischer, Lutz