Cellular model to assess the antioxidant activity of lactobacilli
Cellular model to assess the antioxidant activity of lactobacilli
复制标题
评估乳酸菌抗氧化活性的细胞模型
DOI：
10.1039/c5ra02215k
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发表时间：
2015-04
期刊：
影响因子：
3.9
通讯作者：
Chen, Wei
中科院分区：
文献类型：
--
作者：
Zhao, Jianxin;Zhang, Hao;Chen, Yong Q.;Chen, Wei
乳酸菌具有多种健康保护作用。一个有益的影响是它们的抗氧化活性，这需要使用有效的生物学相关分析来测量。本研究采用细胞抗氧化法（CAA）测定了5种（鼠李糖乳杆菌、植物乳杆菌、嗜酸乳杆菌、干酪乳杆菌、发酵乳杆菌）10株乳酸菌在肝癌（HepG2）细胞中的抗氧化性能，并与传统的2,2-二苯基苦酰酰（DPPH）自由基清除法进行了比较。两种方法的结果在三种浓度（107 ~ 109个菌落形成单位/ mL）下无明显相关性。进一步的研究评估了菌株对h2o2诱导的HepG2细胞氧化应激的保护作用，结果表明CAA法获得的数据比DPPH自由基清除法获得的数据更一致。研究结果表明，CAA可能是检测乳杆菌抗氧化活性的较好选择。
Lactobacilli are associated with multiple health-protective effects. One beneficial effect is their antioxidant activity, which needs to be measured using efficient biologically relevant assays. In this study, a cellular antioxidant assay (CAA) was used to determine the cellular anti-oxidative properties of 10 Lactobacillus strains from 5 species (L. rhamnosus, L. plantarum, L. acidophilus, L. casei, L. fermenti) in hepatocellular carcinoma (HepG2) cells and the results were compared with those obtained with the traditional 2,2-diphenylpicrylhydrazyl (DPPH) radical scavenging assay. The results from these two methods showed no obvious correlation at three concentrations (107–109 colony-forming units per mL). A further study was performed to evaluate the protective effects of the strains against H2O2-induced oxidative stress in HepG2 cells and the results showed greater consistency in the data obtained with CAA assay than in those from the DPPH radical scavenging assay. The findings indicate that CAA may be a better choice for the detection of the antioxidant activity of Lactobacillus strains.
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影响因子：
8.8
作者：
Kabir, Faisal;Tow, Wei Wei;Nakamura, Soichiro
通讯作者：
Nakamura, Soichiro
DOI：
10.1016/j.alcohol.2008.12.009
发表时间：
2009-03
期刊：
Alcohol (Fayetteville, N.Y.)
影响因子：
--
作者：
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通讯作者：
Keshavarzian A
影响因子：
--
作者：
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通讯作者：
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影响因子：
2.4
作者：
Lee, J;Hwang, KT;Park, KY
通讯作者：
Park, KY
影响因子：
8.8
作者：
Huang, Haizhi;Sun, Yujing;Ye, Xingqian
通讯作者：
Ye, Xingqian