Distribution of short interstitial telomere motifs in two plant genomes: putative origin and function.
Distribution of short interstitial telomere motifs in two plant genomes: putative origin and function.
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DOI：
10.1186/1471-2229-10-283
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发表时间：
2010-12-20
影响因子：
5.3
通讯作者：
Lescure B
中科院分区：
文献类型：
--
作者：
Gaspin C;Rami JF;Lescure B
短间隙端粒基序(端粒盒)是与植物端粒重复单位相同的短序列。它们是在周期细胞中几个过度表达的基因的5‘区域内观察到的。在多种顺式作用元件的协同作用下，这些基序参与表达的激活。在这里，我们分析了TELO盒在拟南芥和水稻基因组中的分布及其与参与翻译装置生物发生的基因的关系。我们的分析表明，端粒框(AAACCCTA)在拟南芥和栽培稻不同基因组区域的分布不是随机的。与植物微卫星一样，它们优先位于基因的5‘侧翼区，主要位于5’非编码区内，并沿转录方向呈梯度分布。正如以前在拟南芥中报道的那样，在水稻核糖体蛋白编码基因的几乎所有启动子中，都观察到了末端框与II或TEF顺式作用元件的保守拓扑关联。这种保守的启动子组织可以在参与翻译机制生物发生的其他基因中找到，包括rRNA加工蛋白和snoRNAs。值得注意的是，Telo盒与II基序或TEF盒的关联在含有嵌套在内含子内的snoRNA簇的基因的启动子中以及在非内含子snoRNA基因的5‘侧翼区域中是保守的。因此，寻找TELO盒与植物基因组中的位点II基序或TEF盒之间的关联可以为鉴定新的隐蔽的RNA PolII启动子提供有用的工具。这项工作中报告的数据支持先前提出的在植物基因组中传播TELO盒的模型，并为获得植物微卫星的假定过程提供了新的见解。Telo盒与Site II或TEF顺式作用元件的结合似乎是参与核糖体生物发生的植物基因的一个基本特征，并清楚地表明大多数植物snoRNAs是RNAPolII产物。
Short interstitial telomere motifs (telo boxes) are short sequences identical to plant telomere repeat units. They are observed within the 5' region of several genes over-expressed in cycling cells. In synergy with various cis-acting elements, these motifs participate in the activation of expression. Here, we have analysed the distribution of telo boxes within Arabidopsis thaliana and Oryza sativa genomes and their association with genes involved in the biogenesis of the translational apparatus. Our analysis showed that the distribution of the telo box (AAACCCTA) in different genomic regions of A. thaliana and O. sativa is not random. As is also the case for plant microsatellites, they are preferentially located in the 5' flanking regions of genes, mainly within the 5' UTR, and distributed as a gradient along the direction of transcription. As previously reported in Arabidopsis, a conserved topological association of telo boxes with site II or TEF cis-acting elements is observed in almost all promoters of genes encoding ribosomal proteins in O. sativa. Such a conserved promoter organization can be found in other genes involved in the biogenesis of the translational machinery including rRNA processing proteins and snoRNAs. Strikingly, the association of telo boxes with site II motifs or TEF boxes is conserved in promoters of genes harbouring snoRNA clusters nested within an intron as well as in the 5' flanking regions of non-intronic snoRNA genes. Thus, the search for associations between telo boxes and site II motifs or TEF box in plant genomes could provide a useful tool for characterizing new cryptic RNA pol II promoters. The data reported in this work support the model previously proposed for the spreading of telo boxes within plant genomes and provide new insights into a putative process for the acquisition of microsatellites in plants. The association of telo boxes with site II or TEF cis-acting elements appears to be an essential feature of plant genes involved in the biogenesis of ribosomes and clearly indicates that most plant snoRNAs are RNA pol II products.
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影响因子：
5.1
作者：
LIBOZ, T;BARDET, C;LESCURE, B
通讯作者：
LESCURE, B
影响因子：
5.3
作者：
LEE, MS;BLACKBURN, EH
通讯作者：
BLACKBURN, EH
AthaMap，整合转录和转录后数据。
影响因子：
14.9
作者：
Buelow, Lorenz;Engelmann, Stefan;Schindler, Martin;Hehl, Reinhard
通讯作者：
Hehl, Reinhard
影响因子：
3.5
作者：
Fujimori, S;Washio, T;Tomita, M
通讯作者：
Tomita, M
DOI：
10.1007/bf00292002
发表时间：
1993-04-01
期刊：
MOLECULAR & GENERAL GENETICS
影响因子：
--
作者：
CURIE, C;AXELOS, M;LESCURE, B
通讯作者：
LESCURE, B