Defining the Sphagnum Core Microbiome across the North American Continent Reveals a Central Role for Diazotrophic Methanotrophs in the Nitrogen and Carbon Cycles of Boreal Peatland Ecosystems
Defining the Sphagnum Core Microbiome across the North American Continent Reveals a Central Role for Diazotrophic Methanotrophs in the Nitrogen and Carbon Cycles of Boreal Peatland Ecosystems
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DOI：
10.1128/mbio.03714-21
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发表时间：
2022-02-22
期刊：
影响因子：
6.4
通讯作者：
Kostka JE
中科院分区：
文献类型：
--
作者：
Kolton M;Weston DJ;Mayali X;Weber PK;McFarlane KJ;Pett-Ridge J;Somoza MM;Lietard J;Glass JB;Lilleskov EA;Shaw AJ;Tringe S;Hanson PJ;Kostka JE
泥炭藓属的泥炭苔藓是生态系统工程师，它们经常在北方泥炭地控制光合作用的生产。泥炭草属Sphagnum spp.有多种微生物群落，能够固氮(重氮营养)和甲烷氧化(甲烷营养)，从而潜在地支持植物在严重营养限制条件下的生长。此外，重氮营养的甲烷氧化菌可能是碳和氮循环之间的“缺失环节”，但与泥炭疽菌相关的微生物群的功能贡献仍然存在疑问。应用元基因组学、总转录组学和双同位素掺入分析相结合的方法，研究北美大陆泥炭藓微生物群落组成，为泥炭藓为主的生态系统提供重氮化甲烷营养的经验证据。在美国各地泥炭地(5个州，17个沼泽/沼泽地点，18个泥炭藻种)的250多个泥炭藓SSU rRNA文库中检测到了非常一致的原核生物群落，其中核心微生物组的12个属占相对微生物丰度的60%。此外，固氮酶(NifH)和SSU rRNA基因扩增分析表明，固氮菌群占原核生物群落的近15%，其中以根瘤菌、蓝藻和根瘤菌为主。虽然在扩增和元基因组分析中检测到的重氮菌中，蓝藻占绝大多数(>；95%)，但甲基阿魏酸菌属(根瘤菌目)的专性甲烷营养菌占转录的nifH基因的四分之一。此外，在双同位素示踪实验中，根瘤菌的成员显示13CH4和15N2同位素大量掺入其rRNA中。我们的研究在大的空间尺度上描述了泥炭藓核心微生物群的特征，并指出重氮营养的甲烷营养菌，在这里定义为稀有生物圈的专性甲烷营养菌(甲基阿魏酸菌)。根瘤菌)，也进行重氮营养，在营养贫乏的泥炭地碳和氮循环的耦合中起着关键作用。
Peat mosses of the genus Sphagnum are ecosystem engineers that frequently predominate over photosynthetic production in boreal peatlands. Sphagnum spp. host diverse microbial communities capable of nitrogen fixation (diazotrophy) and methane oxidation (methanotrophy), thereby potentially supporting plant growth under severely nutrient-limited conditions. Moreover, diazotrophic methanotrophs represent a possible “missing link” between the carbon and nitrogen cycles, but the functional contributions of the Sphagnum-associated microbiome remain in question. A combination of metagenomics, metatranscriptomics, and dual-isotope incorporation assays was applied to investigate Sphagnum microbiome community composition across the North American continent and provide empirical evidence for diazotrophic methanotrophy in Sphagnum-dominated ecosystems. Remarkably consistent prokaryotic communities were detected in over 250 Sphagnum SSU rRNA libraries from peatlands across the United States (5 states, 17 bog/fen sites, 18 Sphagnum species), with 12 genera of the core microbiome comprising 60% of the relative microbial abundance. Additionally, nitrogenase (nifH) and SSU rRNA gene amplicon analysis revealed that nitrogen-fixing populations made up nearly 15% of the prokaryotic communities, predominated by Nostocales cyanobacteria and Rhizobiales methanotrophs. While cyanobacteria comprised the vast majority (>95%) of diazotrophs detected in amplicon and metagenome analyses, obligate methanotrophs of the genus Methyloferula (order Rhizobiales) accounted for one-quarter of transcribed nifH genes. Furthermore, in dual-isotope tracer experiments, members of the Rhizobiales showed substantial incorporation of 13CH4 and 15N2 isotopes into their rRNA. Our study characterizes the core Sphagnum microbiome across large spatial scales and indicates that diazotrophic methanotrophs, here defined as obligate methanotrophs of the rare biosphere (Methyloferula spp. of the Rhizobiales) that also carry out diazotrophy, play a keystone role in coupling of the carbon and nitrogen cycles in nutrient-poor peatlands.
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影响因子：
3.7
作者：
Fritz C;Lamers LP;Riaz M;van den Berg LJ;Elzenga TJ
通讯作者：
Elzenga TJ
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