Aberrant Expression of Critical Genes during Secondary Cell Wall Biogenesis in a Cotton Mutant, Ligon Lintless-1 (Li-1).
Aberrant Expression of Critical Genes during Secondary Cell Wall Biogenesis in a Cotton Mutant, Ligon Lintless-1 (Li-1).
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DOI：
10.1155/2009/659301
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发表时间：
2009
影响因子：
--
通讯作者：
Jenkins JN
中科院分区：
文献类型：
--
作者：
Bolton JJ;Soliman KM;Wilkins TA;Jenkins JN
棉花价值的90%以上来自其纤维;然而，对控制纤维发育的遗传机制知之甚少。由于其纤维细胞的生物化学和形态多样性，棉花纤维突变体已被用于检查纤维发育，因此，使用Ligon无绒（L i-1）突变体，一个单基因显性棉花突变体与非常短的纤维，我们采用了高通量的微阵列技术和真实的时间PCR的方法，以获得在次生细胞壁合成阶段的关键基因的见解。正常TM-1基因型和近等基因L i-1的比较转录组分析显示，超过100个转录本在次生壁生物发生过程中差异表达至少2倍，尽管扩增阶段的遗传谱在等值线中没有显着差异。特别值得注意的是，我们确定了三个候选基因家族-expansin，蔗糖合成酶，和微管蛋白-其在L i-1中的表达偏离其亲本TM-1的正常表达模式。这些基因可能有助于延迟纤维生长在L i-1，因为它们是纤维表达的结构和代谢基因。这项工作为纤维发育的机制提供了更多的细节，并表明L i基因在纤维发育的后期阶段是活跃的。
Over ninety percent of the value of cotton comes from its fiber; however, the genetic mechanisms governing fiber development are poorly understood. Due to their biochemical and morphological diversity in fiber cells cotton fiber mutants have been useful in examining fiber development; therefore, using the Ligon Lintless (L i-1) mutant, a monogenic dominant cotton mutant with very short fibers, we employed the high throughput approaches of microarray technology and real time PCR to gain insights into what genes were critical during the secondary cell wall synthesis stage. Comparative transcriptome analysis of the normal TM-1 genotype and the near isogenic L i-1 revealed that over 100 transcripts were differentially expressed at least 2-fold during secondary wall biogenesis, although the genetic profile of the expansion phase showed no significant differences in the isolines. Of particular note, we identified three candidate gene families-expansin, sucrose synthase, and tubulin—whose expression in L i-1 deviates from normal expression patterns of its parent, TM-1. These genes may contribute to retarded growth of fibers in L i-1 since they are fiber-expressed structural and metabolic genes. This work provides more details into the mechanisms of fiber development, and suggests the L i gene is active during the later stages of fiber development.
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影响因子：
2.3
作者：
KOHEL, RJ;BENEDICT, CR;JIVIDEN, GM
通讯作者：
JIVIDEN, GM
影响因子：
11.6
作者：
Pastuglia, Martine;Azimzadeh, Juliette;Bouchez, David
通讯作者：
Bouchez, David
影响因子：
3.1
作者：
An, Chuanfu;Saha, Sukumar;Stelly, David M.
通讯作者：
Stelly, David M.
影响因子：
2.3
作者：
KOHEL, RJ;QUISENBE.JE;BENEDICT, CR
通讯作者：
BENEDICT, CR
DOI：
10.1083/jcb.141.1.163
发表时间：
1998-04-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Dictenberg JB;Zimmerman W;Sparks CA;Young A;Vidair C;Zheng Y;Carrington W;Fay FS;Doxsey SJ
通讯作者：
Doxsey SJ