A ratiometric fluorescent nanoprobe for signal amplification monitoring of intracellular telomerase activity
A ratiometric fluorescent nanoprobe for signal amplification monitoring of intracellular telomerase activity
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用于信号放大监测细胞内端粒酶活性的比例荧光纳米探针
DOI：
10.1007/s00216-021-03823-5
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发表时间：
2022-01
影响因子：
4.3
通讯作者：
Congxiu Miao
中科院分区：
文献类型：
--
作者：
D;an Ma;Huiyun Bai;Junbo Li;Yintao Li;Lihua Song;Jinping Zheng;Congxiu Miao
端粒酶被认为是一种有价值的诊断和预后癌症的生物标志物。准确可靠地检测端粒酶活性对临床诊断、抑制剂筛选和治疗具有重要价值。在这里，我们开发了一种新型的放大荧光共振能量转移（FRET）纳米探针，用于高灵敏度和可靠的监测细胞内端粒酶活性。纳米探针（QDSA@DNA）由链霉亲和素修饰的量子点（QDSA）组成，所述量子点通过生物素-链霉亲和素相互作用用端粒酶引物序列（TP）和Cy 5标记的信号转换序列（SS）官能化。当组装纳米探针时，Cy 5非常接近QDSA，导致从QDSA到Cy 5的高FRET效率。在端粒酶的作用下，TP可以延伸产生端粒重复单元，与SS的环互补。因此，SS可以与延长的序列杂交以形成刚性双链结构，这迫使Cy 5远离QDSA的表面，导致低FRET效率。此外，由于端粒酶产生多个重复单元，可以打开多个发夹结构，产生显著的荧光比率（Δ Dsa/FCy 5）增强，用于检测端粒酶活性。以这种方式，在纳米探针中FRET和靶辅助策略的组合分别提高了检测精度和放大了检测信号。QDSA@DNA纳米探针还显示出高选择性、优异的核酸酶稳定性和良好的生物相容性。更重要的是，这种纳米探针被发现是一种有效监测细胞内端粒酶活性的极好平台，为肿瘤诊断和端粒酶相关抑制剂的筛选提供了潜在的平台。
Telomerase is considered a valuable diagnostic and prognostic cancer biomarker. Accurate and reliable detection of telomerase activity is of great value in clinical diagnosis, screening of inhibitors, and therapeutics. Here, we developed a novel amplified fluorescence resonance energy transfer (FRET) nanoprobe for highly sensitive and reliable monitoring of intracellular telomerase activity. The nanoprobe (QDSA@DNA) was composed of a streptavidin-modified quantum dot (QDSA) which was functionalized with a telomerase primer sequence (TP) and Cy5-tagged signal switching sequence (SS) through biotin-streptavidin interaction. When the nanoprobe was assembled, the Cy5 was in close proximity to the QDSA, resulting in high FRET efficiency from the QDSAto Cy5. In the presence of telomerase, the TP could be extended to produce telomeric repeat units, which was complementary to the loop of SS. Thus, the SS could hybridize with elongated sequences to form a rigid double-stranded structure, which forced the Cy5 away from the surface of the QDSA, causing low FRET efficiency. Furthermore, due to the production of multiple repeat units by telomerase, multiple hairpin structures could be opened, yielding significant fluorescence ratio (FQDsa/FCy5) enhancement for sensing of telomerase activity. In this way, the combination of a FRET and target-assisted strategy in a nanoprobe improved the detection accuracy and amplified the detection signal, respectively. The QDSA@DNA nanoprobe also showed high selectivity, excellent nuclease stability, and good biocompatibility. More importantly, this nanoprobe was found to be an excellent platform for efficient monitoring of intracellular telomerase activity, providing a potential platform in tumor diagnosis and screening of telomerase-related inhibitors.Graphical abstract
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影响因子：
7.4
作者：
Di Su;Xiangyi Huang;Chaoqing Dong;Jicun Ren
通讯作者：
Di Su;Xiangyi Huang;Chaoqing Dong;Jicun Ren
通过自组装策略将水溶性疏水性 ZnAgInSe/ZnS QD 与肿瘤靶向 cRGD-磺基甜菜碱-PIMA-组胺配体结合用于生物成像
影响因子：
9.5
作者：
Deng, Tao;Peng, Yanan;Deng, Dawei
通讯作者：
Deng, Dawei
影响因子：
6.1
作者：
T. Yu;Wei Zhao;Jingjuan Xu;Hongyuan Chen
通讯作者：
T. Yu;Wei Zhao;Jingjuan Xu;Hongyuan Chen
DOI：
10.1021/acs.jpclett.7b01259
发表时间：
2017-07
期刊：
The journal of physical chemistry letters
影响因子：
--
作者：
S. Kaniyankandy;S. Verma
通讯作者：
S. Kaniyankandy;S. Verma
影响因子：
4.7
作者：
Narendra Pratap Singh;S. Charan;K. Sanjiv;Shih-Hsin Huang;Yu-Chu Hsiao;C. Kuo;F. Chien;Terrence C Lee-Terr
通讯作者：
Narendra Pratap Singh;S. Charan;K. Sanjiv;Shih-Hsin Huang;Yu-Chu Hsiao;C. Kuo;F. Chien;Terrence C Lee-Terr