Electroacupuncture Improves Cognitive Function in Senescence-Accelerated P8 (SAMP8) Mice via the NLRP3/Caspase-1 Pathway.
Electroacupuncture Improves Cognitive Function in Senescence-Accelerated P8 (SAMP8) Mice via the NLRP3/Caspase-1 Pathway.
复制标题
电针通过 NLRP3/Caspase-1 通路改善衰老加速 P8 (SAMP8) 小鼠的认知功能
DOI：
10.1155/2020/8853720
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发表时间：
2020
影响因子：
3.1
通讯作者：
Sun Z
中科院分区：
文献类型：
--
作者：
Hou Z;Qiu R;Wei Q;Liu Y;Wang M;Mei T;Zhang Y;Song L;Shao X;Shang H;Chen J;Sun Z
背景临床上，电针（EA）是治疗与年龄相关的认知障碍（CI）的最常见疗法。然而，潜在的病理机制仍然不明。本研究旨在观察电针对认知功能的影响，并探讨电针对NLRP 3/caspase-1信号通路的作用机制。主要方法将30只SAMP 8雄性小鼠随机分为模型组、2 Hz电针组和10 Hz电针组。将10只雄性SAMR 1小鼠分配至对照组。通过Morris水迷宫测试评估认知功能。HE染色观察海马组织形态学变化，TUNEL染色观察海马细胞凋亡情况。ELISA法检测血清IL-1β、IL-6、IL-18和TNF-α水平。Western blotting检测海马NLRP 3、ASC、caspase-1、GSDM-D、IL-1β、IL-18、Aβ和tau蛋白。主要发现。认知功能，海马形态，TUNEL阳性细胞计数改善两个EA频率。电针可降低血清IL-1β、IL-6、IL-18和TNF-α水平。10 Hz电针组较2 Hz电针组能更有效地降低海马CA 1区TUNEL阳性细胞数和血清IL-1β、IL-6水平。电针可显著下调NLRP 3/caspase-1通路相关蛋白的表达，但2 Hz电针不能有效降低ASC蛋白的表达。有趣的是，两种电针频率均未能降低Aβ和tau蛋白的表达。意义电针GV 20和ST 36穴对NLRP 3/caspase-1信号通路的影响可能是电针缓解衰老相关脑梗死的机制之一。
Background. Clinically, electroacupuncture (EA) is the most common therapy for aging-related cognitive impairment (CI). However, the underlying pathomechanism remains unidentified. The aims of this study were to observe the effect of EA on cognitive function and explore the potential mechanism by which EA acts on the NLRP3/caspase-1 signaling pathway. Main Methods. Thirty male SAMP8 mice were randomly divided into the model, the 2 Hz EA and 10 Hz EA groups. Ten male SAMR1 mice were assigned to the control group. Cognitive function was assessed through the Morris water maze test. Hippocampal morphology and cell death were observed by HE and TUNEL staining, respectively. The serum IL-1β, IL-6, IL-18, and TNF-α levels were measured by ELISA. Hippocampal NLRP3, ASC, caspase-1, GSDM-D, IL-1β, IL-18, Aβ, and tau proteins were detected by Western blotting. Key Findings. Cognitive function, hippocampal morphology, and TUNEL-positive cell counts were improved by both EA frequencies. The serum IL-1β, IL-6, IL-18, and TNF-α levels were decreased by EA treatment. However, 10 Hz EA reduced the number of TUNEL-positive cells in the CA1 region and serum IL-1β and IL-6 levels more effectively than 2 Hz EA. NLRP3/caspase-1 pathway-related proteins were significantly downregulated by EA, but 2 Hz EA did not effectively reduce ASC protein expression. Interestingly, both EA frequencies failed to reduce the expression of Aβ and tau proteins. Significance. The effects of 10 Hz EA at the GV20 and ST36 acupoints on the NLRP3/caspase-1 signaling pathway may be a mechanism by which this treatment relieves aging-related CI in mice.
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影响因子：
8.1
作者：
Nakanishi A;Kaneko N;Takeda H;Sawasaki T;Morikawa S;Zhou W;Kurata M;Yamamoto T;Akbar SMF;Zako T;Masumoto J
通讯作者：
Masumoto J
影响因子：
2.4
作者：
Overton, Marieclaire;Pihlsgard, Mats;Elmstahl, Solve
通讯作者：
Elmstahl, Solve
DOI：
10.1155/2020/7190495
发表时间：
2020-02-26
影响因子：
--
作者：
Lin, Ruizhu;Huang, Jia;Chen, Lidian
通讯作者：
Chen, Lidian
影响因子：
4
作者：
Seblova, Dominika;Brayne, Carol;Cermakova, Pavla
通讯作者：
Cermakova, Pavla
高碳酸血症通过激活 NLRP3 炎性体诱导 IL-1 β 过量产生：对低氧成年大鼠认知障碍的影响
影响因子：
9.3
作者：
Ding HG;Deng YY;Yang RQ;Wang QS;Jiang WQ;Han YL;Huang LQ;Wen MY;Zhong WH;Li XS;Yang F;Zeng HK
通讯作者：
Zeng HK