Porcine reproductive-respiratory syndrome virus infection predisposes pigs for respiratory signs upon exposure to bacterial lipopolysaccharide.
Porcine reproductive-respiratory syndrome virus infection predisposes pigs for respiratory signs upon exposure to bacterial lipopolysaccharide.
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DOI：
10.1016/s0378-1135(02)00104-9
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发表时间：
2002-08-02
影响因子：
3.3
通讯作者：
Pensaert M
中科院分区：
文献类型：
--
作者：
Labarque G;Van Reeth K;Van Gucht S;Nauwynck H;Pensaert M
本研究探讨了是否感染猪繁殖与呼吸综合征病毒（PRRSV）加强呼吸道症状后，暴露于细菌脂多糖（LPS）。用PRRSV的莱利斯塔株对5周龄的常规猪进行肠道内接种，并在5天后接受一次或两次肠道内LPS施用。包括必要的控制。LPS给药后，密切监测猪的临床体征。此外，在第二次LPS给药后对一些猪实施安乐死，以进行支气管肺泡细胞分析和肺部病毒学检查。对支气管肺泡灌洗液（BAL）细胞进行计数和分化。滴定肺悬液和BAL液中的PRRSV。猪暴露于PRRSV仅导致发热1至5天，并出现轻微呼吸道症状。猪暴露于LPS仅导致以发热和抑郁为特征的一般体征，但呼吸体征轻微或不存在。另一方面，PRRSV-LPS暴露的猪在LPS暴露后出现严重的呼吸体征，特征为呼吸急促、腹式呼吸和呼吸困难。除了呼吸道症状外，这些猪还表现出增强的一般症状，如发热和抑郁。肺中性粒细胞浸润是相似的，在非感染和PRRSV感染的猪LPS暴露。在仅感染PRRSV的猪和暴露于PRRSV-LPS的猪的肺和BAL液中，PRRSV数量相似。这些数据显示了PRRSV和LPS在常规猪中诱导呼吸体征方面的明显协同作用。在87%的猪中观察到协同作用。因此，它可以被认为是可重复的，并可用于测试预防和治疗措施的有效性。
This study examined whether an infection with porcine reproductive and respiratory syndrome virus (PRRSV) potentiates respiratory signs upon exposure to bacterial lipopolysaccharides (LPS). Five-week-old conventional pigs were inoculated intratracheally with the Lelystad strain of PRRSV and received 5 days later one or two intratracheal LPS administrations. The necessary controls were included. After LPS administration, pigs were intensively monitored for clinical signs. Additionally, some pigs were euthanatized after a second LPS administration for broncho-alveolar cell analysis and virological examinations of the lungs. Broncho-alveolar lavage (BAL) cells were counted and differentiated. Lung suspensions and BAL fluids were titrated for PRRSV. Exposure of pigs to PRRSV only resulted in a fever for time periods ranging from 1 to 5 days and slight respiratory signs. Exposure of pigs to LPS only resulted in general signs, characterized by fever and depression, but respiratory signs were slight or absent. PRRSV–LPS exposed pigs, on the other hand, developed severe respiratory signs upon LPS exposure, characterized by tachypnoea, abdominal breathing and dyspnoea. Besides respiratory signs, these pigs also showed enhanced general signs, such as fever and depression. Lung neutrophil infiltration was similar in non-infected and PRRSV-infected pigs upon LPS exposure. PRRSV quantities were similar in lungs and BAL fluids of pigs infected with PRRSV only and PRRSV–LPS exposed pigs. These data show a clear synergism between PRRSV and LPS in the induction of respiratory signs in conventional pigs. The synergism was observed in 87% of the pigs. So, it can be considered as reproducible and may be used to test the efficacy of preventive and therapeutic measures.
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影响因子：
3.3
作者：
Wills, RW;Gray, JT;Zimmerman, JJ
通讯作者：
Zimmerman, JJ
影响因子：
2.2
作者：
ALBINA, E;MADEC, F;TORRISON, J
通讯作者：
TORRISON, J
影响因子：
1
作者：
Baarsch, MJ;Foss, DL;Murtaugh, MP
通讯作者：
Murtaugh, MP
影响因子：
3.3
作者：
VanReeth, K;Nauwynck, H;Pensaert, M
通讯作者：
Pensaert, M
影响因子：
4.9
作者：
Lamp, KC;Rybak, MJ;Summers, KK
通讯作者：
Summers, KK