Polyvalent protective immunogens identified from outer membrane proteins of Vibrio parahaemolyticus and their induced innate immune response
Polyvalent protective immunogens identified from outer membrane proteins of Vibrio parahaemolyticus and their induced innate immune response
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从副溶血弧菌外膜蛋白中鉴定出的多价保护性免疫原及其诱导的先天免疫反应
DOI：
10.1016/j.fsi.2017.10.046
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Peng B;Lin XP;Wang SN;Yang MJ;Peng XX;Li H
疫苗是保护宿主免受细菌感染的最经济、最有效、最环保的制剂。在水产养殖中，由于养殖环境中存在各种类型的细菌病原体，对针对多种细菌的多价疫苗的需求很高。克隆并表达了18个编码溶血性弧菌外膜蛋白的基因。将表达的重组蛋白用于制备抗血清。在斑马鱼模型上研究了抗血清和重组蛋白的被动和主动免疫保护作用。两种重组蛋白VP1667和VP2369对至少两个属的细菌显示出有效的免疫保护作用，包括副溶血性弧菌和副溶血性弧菌。溶藻杆菌)、荧光假单胞菌或/和嗜水气单胞菌，因此是潜在的多价疫苗候选者，可用于预防养鱼业的细菌感染。此外，还探讨了两种多价疫苗诱导免疫应答的机制。VP1667或VP2369抗血清与P无交叉反应。荧光素A。而这两种重组蛋白都能诱导显著的先天免疫反应。相比之下，VP1667刺激较强的淋巴因子和单核细胞因子，VP2369诱导较强的体液免疫应答，而两者产生相似的NF-κB、COX-2、TLR1和TLR3表达。我们的结果识别了两种多价疫苗，并展示了它们在天然免疫反应内容上的交叉保护特征。
Vaccines are the most economic, efficient and environment-friendly agents in protecting host against bacterial infection. In aquaculture, polyvalent vaccines targeting more than one bacterial specie are highly demanded due to the presence of various types of bacterial pathogens in farming environment. Here eighteen genes encoding outer membrane proteins of Vibrioparahaemolyticuswere cloned and expressed. The expressed recombinant proteins were used for antiserum preparation. Passive and active immune protection of the antiserum and recombinant proteins was investigated in the zebrafish model. Two recombinant proteins, VP1667 and VP2369, showed effective immune protection against at least two genera of bacteria, Vibrio (V. parahaemolyticusandV. alginolyticus), Pseudomonas(P. fluorescens)or/and Aeromonas (A. hydrophila), and thereby are potential polyvalent vaccine candidates to defend against bacterial infection in fish farming. Furthermore, the mechanisms for the two polyvalent vaccines in triggering immune response were explored. Antiserum to VP1667 or VP2369 was not cross-reacted withP. fluorescensandA. hydrophila,whereas both recombinant proteins induced significant innate immune response. Comparatively, VP1667 stimulates stronger lymphokine and monokine, and VP2369 induces stronger humoral immune response, while both produce similar NF-κB, COX-2, TLR-1 and TLR-3 expression. Our results identify two polyvalent vaccines and demonstrate characteristics features of their cross-protection at the content of the innate immune response.
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DOI：
10.1016/0140-6736(90)92435-k
发表时间：
1950-05
期刊：
The Yale Journal of Biology and Medicine
影响因子：
--
作者：
S. Eykyn
通讯作者：
S. Eykyn
影响因子：
4.7
作者：
Hui Li;Xiao Chu;Bo Peng;Xuan-xian Peng
通讯作者：
Hui Li;Xiao Chu;Bo Peng;Xuan-xian Peng
影响因子：
4.3
作者：
Shen, Qing;Yang, Qi;Cheung, Hon-Yeung
通讯作者：
Cheung, Hon-Yeung
影响因子：
4.7
作者：
Hu, Zhenyu;Wu, Bingqian;Zhao, Heng
通讯作者：
Zhao, Heng
暴露于金霉素的浮游嗜水气单胞菌的整体蛋白表达谱响应
影响因子：
4.1
作者：
Li, Wanxin;Yao, Zujie;Lin, Xiangmin
通讯作者：
Lin, Xiangmin