Co-culture with infrapatellar fat pad differentially stimulates proteoglycan synthesis and accumulation in cartilage and meniscus tissues.
Co-culture with infrapatellar fat pad differentially stimulates proteoglycan synthesis and accumulation in cartilage and meniscus tissues.
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DOI：
10.1080/03008207.2016.1245728
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发表时间：
2017-09
影响因子：
2.9
通讯作者：
Levenston ME
中科院分区：
文献类型：
--
作者：
Nishimuta JF;Bendernagel MF;Levenston ME
尽管骨关节炎被广泛认为是一种全关节疾病，但相对较少的研究关注关节组织在关节稳态和退变中的相互作用。特别是，很少有研究检查髌下脂肪垫（IFP）对软骨组织的影响。本研究的目的是验证与健康IFP共培养会诱导软骨和半月板组织退化的假设。牛关节软骨、半月板和IFP分别单独培养或作为软骨-脂肪或半月板-脂肪共培养达14天。条件培养基测定硫酸糖胺聚糖（sGAG）含量、亚硝酸盐含量和基质金属蛋白酶（MMP）活性，外植体测定sGAG和DNA含量。软骨和半月板的sGAG累积释放量和sGAG释放率均有所增加，而软骨（半月板除外）在共培养中表现出实质性的协同效应（共培养中的sGAG释放量显著大于分离软骨和脂肪的总释放量）。脂肪共培养没有显著改变软骨或半月板外植体的sGAG含量，这表明IFP共培养刺激了软骨的sGAG净产量。与分离组织对照相比，共培养的半月板中亚硝酸盐释放量增加，而软骨中则没有，共培养无协同效应。有趣的是，软骨和半月板的共培养减少了MMP-2的产生。本研究表明，健康的IFP可能通过刺激软骨中sGAG的产生来调节关节的内稳态。与我们的假设相反，健康的IFP不会促进软骨或半月板组织的退化。
Although osteoarthritis is widely viewed as a disease of the whole joint, relatively few studies have focused on interactions among joint tissues in joint homeostasis and degeneration. In particular, few studies have examined effects of the infrapatellar fat pad (IFP) on cartilaginous tissues. The aim of this study was to test the hypothesis that co-culture with healthy IFP would induce degradation of cartilage and meniscus tissues. Bovine articular cartilage, meniscus, and IFP were cultured isolated or as cartilage-fat or meniscus-fat co-cultures for up to 14 days. Conditioned media were assayed for sulfated glycosaminoglycan (sGAG) content, nitrite content and matrix metalloproteinase (MMP) activity, and explants were assayed for sGAG and DNA contents. Co-cultures exhibited increased cumulative sGAG release and sGAG release rates for both cartilage and meniscus, and cartilage (but not meniscus) exhibited a substantial synergestic effect of co-culture (sGAG release in co-culture was significantly greater than the summed release from isolated cartilage and fat). Fat co-culture did not significantly alter sGAG content of either cartilage or meniscus explants, indicating that IFP co-culture stimulated net sGAG production by cartilage. Nitrite release was increased relative to isolated tissue controls in co-cultured meniscus but not cartilage, with no synergistic effect of co-culture. Interestingly, MMP-2 production was decreased by co-culture for both cartilage and meniscus. This study demonstrates that healthy IFP may modulate joint homeostasis by stimulating sGAG production in cartilage. Counter to our hypothesis, healthy IFP did not promote degradation of either cartilage or meniscus tissue.
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影响因子：
4.9
作者：
Ioan-Facsinay A;Kloppenburg M
通讯作者：
Kloppenburg M
影响因子：
7
作者：
Nishimuta, J. F.;Levenston, M. E.
通讯作者：
Levenston, M. E.
影响因子：
2.8
作者：
Ling, Carrie H-Y;Lai, Janice H.;Levenston, Marc E.
通讯作者：
Levenston, Marc E.
影响因子：
13.3
作者：
Eymard, Florent;Pigenet, Audrey;Houard, Xavier
通讯作者：
Houard, Xavier
影响因子：
--
作者：
Beekhuizen, Michiel;Bastiaansen-Jenniskens, Yvonne M.;van Osch, Gerjo J. V. M.
通讯作者：
van Osch, Gerjo J. V. M.