Response of cartilage and meniscus tissue explants to in vitro compressive overload.
Response of cartilage and meniscus tissue explants to in vitro compressive overload.
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DOI：
10.1016/j.joca.2012.01.004
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发表时间：
2012-05
影响因子：
7
通讯作者：
Levenston, M. E.
中科院分区：
文献类型：
--
作者：
Nishimuta, J. F.;Levenston, M. E.
通过施加单一、受控的过载并观察细胞、生化和机械变化，检查软骨和半月板组织对机械损伤的相对易感性。通过在三种不同的应变速率下压痕至40%应变，使径向限制中的腕关节和半月板组织外植体受到一系列损伤：0.5%/s（慢）、5%/s（中）或50%/s（快）。损伤后，将样品培养1天或9天。测定外植体的细胞代谢活性、水含量和sGAG含量。在扭剪和无侧限压缩条件下测定外植体的力学性能。测定条件培养基的sGAG和LDH释放。峰值损伤力随应变率增加而增加，但两种组织几乎没有肉眼可见的损伤。细胞代谢在第1天在中、快组中最低。两种组织的细胞溶解均随峰值损伤力和加载速率的增加而增加。相比之下，sGAG含量和释放不随加载速率显著变化。此外，两种组织的力学性能均未随加载速率发生显著变化。通过使用定制的限制室，获得了大的峰值力，而没有对外植体造成宏观破坏。在本研究中达到的负荷下，诱导细胞损伤，而没有可检测到的物理或成分变化。这些结果表明，亚失效损伤可以诱导生物损伤，可能不容易检测到，可能是骨关节炎发生的早期事件。
To examine the relative susceptibility of cartilage and meniscus tissues to mechanical injury by applying a single, controlled overload and observing cellular, biochemical, and mechanical changes. Cartilage and meniscus tissue explants in radial confinement were subjected to a range of injury by indenting to 40% strain at three different strain rates: 0.5%/s (slow), 5%/s (medium), or 50%/s (fast). Following injury, samples were cultured for either 1 or 9 days. Explants were assayed for cell metabolic activity, water content, and sGAG content. Mechanical properties of explants were determined in torsional shear and unconfined compression. Conditioned medium was assayed for sGAG and LDH release. Peak injury force increased with strain rate but both tissues displayed little to no macroscopic damage. Cell metabolism was lowest in medium and fast groups on day 1. Cell lysis increased with peak injury force and loading rate in both tissues. In contrast, sGAG content and release did not significantly vary with loading rate. Additionally, mechanical properties did not significantly vary with loading rate in either tissue. By use of a custom confinement chamber, large peak forces were obtained without macroscopic destruction of the explants. At the loads achieved in this studied, cell damage was induced without detectable physical or compositional changes. These results indicate that sub-failure injury can induce biologic damage that may not be readily detected and could be an early event in osteoarthritis genesis.
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影响因子：
--
作者：
DiMicco, MA;Patwari, P;Grodzinsky, AJ
通讯作者：
Grodzinsky, AJ
DOI：
10.1016/0304-4165(86)90306-5
发表时间：
1986-09-04
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
FARNDALE, RW;BUTTLE, DJ;BARRETT, AJ
通讯作者：
BARRETT, AJ
影响因子：
5
作者：
DAVIS, MA;ETTINGER, WH;HAUCK, WW
通讯作者：
HAUCK, WW
影响因子：
2.4
作者：
Bursac, PM;Obitz, TW;Stamenovic, D
通讯作者：
Stamenovic, D
影响因子：
3.9
作者：
Kisiday, John D.;Vanderploeg, Eric J.;Frisbie, David D.
通讯作者：
Frisbie, David D.