Regional sex differences in neurochemical profiles of healthy mice measured by magnetic resonance spectroscopy at 9.4 tesla.
Regional sex differences in neurochemical profiles of healthy mice measured by magnetic resonance spectroscopy at 9.4 tesla.
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DOI：
10.3389/fnins.2023.1278828
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发表时间：
2023
影响因子：
4.3
通讯作者：
Oz, Gulin
中科院分区：
文献类型：
--
作者：
Tkac, Ivan;Xie, Tiankai;Shah, Nitya;Larson, Sarah;Dubinsky, Janet M.;Gomez-Pastor, Rocio;McLoughlin, Hayley S.;Orr, Harry T.;Eberly, Lynn E.;Oz, Gulin
在神经退行性疾病模型常用的遗传背景下，确定通过健康小鼠体内质子磁共振波谱（1H MRS）测量的神经化学物质浓度的性别差异。在这项回顾性分析中使用了7项先前研究中从具有C57 BL/6或相关遗传背景的野生型小鼠中收集的1H MRS数据。为了纳入，必须使用先进的1H MRS方案在9.4特斯拉磁场下收集数据，使用异氟烷麻醉和类似的动物处理方案，并且必须提供来自雄性和雌性小鼠的相似数量的数据集用于分析的大脑区域。总体而言，155个光谱从雌性小鼠和166个光谱从雄性小鼠（共321），收集了6个脑区（脑干，小脑，皮质，海马，下丘脑，纹状体）在不同的年龄。在大多数脑区，雄性小鼠的牛磺酸、总肌酸（肌酸+磷酸肌酸）、抗坏血酸盐、葡萄糖和谷氨酸盐浓度始终高于雌性小鼠。纹状体是一个例外，雄性和雌性小鼠的总肌酸相似。下丘脑的性别差异模式与其他区域有显著差异。性别和年龄之间的相互作用是显着的总肌酸和牛磺酸在小脑和海马。区域神经化学水平的性别差异很小，但显著且依赖于年龄，在大多数大脑区域都存在一致的男女差异。神经内分泌区下丘脑显示出不同的模式的性别差异的神经化学水平。能量代谢和细胞密度的差异可能是差异的基础，女性的代谢率较高，男性的能量调节和抗氧化能力较高。
To determine sex differences in the neurochemical concentrations measured by in vivo proton magnetic resonance spectroscopy (1H MRS) of healthy mice on a genetic background commonly used for neurodegenerative disease models. 1H MRS data collected from wild type mice with C57BL/6 or related genetic backgrounds in seven prior studies were used in this retrospective analysis. To be included, data had to be collected at 9.4 tesla magnetic field using advanced 1H MRS protocols, with isoflurane anesthesia and similar animal handling protocols, and a similar number of datasets from male and female mice had to be available for the brain regions analyzed. Overall, 155 spectra from female mice and 166 spectra from male mice (321 in total), collected from six brain regions (brainstem, cerebellum, cortex, hippocampus, hypothalamus, and striatum) at various ages were included. Concentrations of taurine, total creatine (creatine + phosphocreatine), ascorbate, glucose and glutamate were consistently higher in male vs. female mice in most brain regions. Striatum was an exception with similar total creatine in male and female mice. The sex difference pattern in the hypothalamus was notably different from other regions. Interaction between sex and age was significant for total creatine and taurine in the cerebellum and hippocampus. Sex differences in regional neurochemical levels are small but significant and age-dependent, with consistent male–female differences across most brain regions. The neuroendocrine region hypothalamus displays a different pattern of sex differences in neurochemical levels. Differences in energy metabolism and cellular density may underlie the differences, with higher metabolic rates in females and higher osmoregulatory and antioxidant capacity in males.
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影响因子：
4.4
作者：
Haedel, Sven;Wirth, Christoph;Schubert, Florian
通讯作者：
Schubert, Florian
影响因子：
3.3
作者：
KLOSE, U
通讯作者：
KLOSE, U
影响因子：
2.2
作者：
Garwood, M;DelaBarre, L
通讯作者：
DelaBarre, L
影响因子：
4.7
作者：
Emir UE;Brent Clark H;Vollmers ML;Eberly LE;Öz G
通讯作者：
Öz G
影响因子：
11.3
作者：
ANDREASON, PJ;ZAMETKIN, AJ;COHEN, RM
通讯作者：
COHEN, RM