The effect of hypoxia on the proliferation capacity of dermal papilla cell by regulating lactate dehydrogenase.
The effect of hypoxia on the proliferation capacity of dermal papilla cell by regulating lactate dehydrogenase.
复制标题
缺氧调节乳酸脱氢酶对真皮乳头细胞增殖能力的影响
DOI：
10.1111/jocd.13578
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发表时间：
2021-03
影响因子：
2.3
通讯作者：
Lin B
中科院分区：
文献类型：
--
作者：
Ye J;Tang X;Long Y;Chu Z;Zhou Q;Lin B
Hypoxia is of great significance for stem cells to maintain the proliferation and differentiation capacity. As a specialized mesenchymal component of the hair follicle (HF), the dermal papilla cell (DPC) not only regulates HF cycle, but also plays a pivotal role in differentiating hair follicle stem cell(HFSC) into HF. However, whether hypoxia could affect DPCs on proliferation or metabolism remains unclear. In our study, DPCs were cultured in normoxia (20%O2) or hypoxia (5%O2). Cell viability assays were performed, and lactate dehydrogenase (LDH) activity and lactate level in DPCs were detected. After that, LDH was overexpressed or knocked down in DPCs; then, the expression of protein markers (ALP, Ki‐67) was assessed by Western blotting, and cell proliferation was also detected after overexpression or knockdown of LDH. Hypoxia did show positive effect on proliferation of DPCs. The LDH activity of DPCs cultured under hypoxic condition was significantly higher than that of cultured under normoxic condition. Overexpression of LDH significantly up‐regulates the expression of ALP and Ki‐67 compared with knockdown and negative control. Cell proliferation was also promoted in DPCs with elevated LDH. Our findings showed that the proliferation activity of DPCs could be stimulated under hypoxia. Meanwhile, LDH plays an important role in maintaining the activity of DPCs in hypoxic condition.
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影响因子：
21.3
作者：
Flores A;Schell J;Krall AS;Jelinek D;Miranda M;Grigorian M;Braas D;White AC;Zhou JL;Graham NA;Graeber T;Seth P;Evseenko D;Coller HA;Rutter J;Christofk HR;Lowry WE
通讯作者：
Lowry WE
影响因子：
5.3
作者：
Mazumdar J;Dondeti V;Simon MC
通讯作者：
Simon MC
影响因子：
2.5
作者：
Iida, Machiko;Ihara, Setsunosuke;Matsuzaki, Takashi
通讯作者：
Matsuzaki, Takashi
影响因子：
--
作者：
Osada, Aki;Iwabuchi, Tokuro;Okochi, Hitoshi
通讯作者：
Okochi, Hitoshi
影响因子：
21.3
作者：
Fernandes, KJL;McKenzie, IA;Miller, FD
通讯作者：
Miller, FD