Gene expression in periodontal tissues following treatment.
Gene expression in periodontal tissues following treatment.
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DOI：
10.1186/1755-8794-1-30
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发表时间：
2008-07-07
影响因子：
2.7
通讯作者：
Flemmig TF
中科院分区：
文献类型：
--
作者：
Beikler T;Peters U;Prior K;Eisenacher M;Flemmig TF
在牙周炎中，旨在控制牙周生物膜感染的治疗可消除炎症的临床和组织学体征。尽管治疗后牙周组织中发现的细胞类型已得到很好的描述，但有关基因表达的信息仅限于少数候选基因。因此，本研究的目的是确定牙周治疗后严重慢性牙周炎部位牙周组织中免疫和炎症基因的表达谱，以鉴定参与组织稳态的基因。在非手术牙周治疗后六至八周，对 12 名患有严重慢性牙周炎的患者和 11 名健康对照者进行了牙龈活检。使用永生化人角质形成细胞系 (HaCaT) 的 RNA 作为内标。使用专注于炎症相关基因的市售微阵列系统对总RNA进行基因表达谱分析。通过实时 PCR 进行所选基因的事后确认。在分析的 136 个基因中，与健康对照相比，表达最强的 5% 基因是 Interleukin-12A (IL-12A)、Versacan (CSPG-2)、基质金属蛋白酶-1 (MMP-1)、唐氏综合症关键区域蛋白-1 (DSCR-1)、巨噬细胞炎症蛋白-2β (Cxcl-3)、凋亡抑制剂蛋白-1 (BIRC-1)、分化抗原簇 38 (CD38)、G 蛋白信号传导调节因子 1 (RGS-1) 和芬克尔-比斯基斯-金金斯鼠骨肉瘤病毒癌基因 (C-FOS)； 5% 表达强度最低的基因是受体相互作用丝氨酸/苏氨酸激酶-2 (RIP-2)、补体成分 3 (C3)、前列腺素内过氧化物合酶-2 (COX-2)、白介素-8 (IL-8)、内皮素-1 (EDN-1)、纤溶酶原激活剂抑制剂 2 型 (PAI-2)、基质金属蛋白酶-14 (MMP-14) 和干扰素调节因子 7 (IRF-7)。治疗后牙周组织中发现的基因表达谱表明调节组织损伤和修复的途径被激活。
In periodontitis, treatment aimed at controlling the periodontal biofilm infection results in a resolution of the clinical and histological signs of inflammation. Although the cell types found in periodontal tissues following treatment have been well described, information on gene expression is limited to few candidate genes. Therefore, the aim of the study was to determine the expression profiles of immune and inflammatory genes in periodontal tissues from sites with severe chronic periodontitis following periodontal therapy in order to identify genes involved in tissue homeostasis. Gingival biopsies from 12 patients with severe chronic periodontitis were taken six to eight weeks following non-surgical periodontal therapy, and from 11 healthy controls. As internal standard, RNA of an immortalized human keratinocyte line (HaCaT) was used. Total RNA was subjected to gene expression profiling using a commercially available microarray system focusing on inflammation-related genes. Post-hoc confirmation of selected genes was done by Realtime-PCR. Out of the 136 genes analyzed, the 5% most strongly expressed genes compared to healthy controls were Interleukin-12A (IL-12A), Versican (CSPG-2), Matrixmetalloproteinase-1 (MMP-1), Down syndrome critical region protein-1 (DSCR-1), Macrophage inflammatory protein-2β (Cxcl-3), Inhibitor of apoptosis protein-1 (BIRC-1), Cluster of differentiation antigen 38 (CD38), Regulator of G-protein signalling-1 (RGS-1), and Finkel-Biskis-Jinkins murine osteosarcoma virus oncogene (C-FOS); the 5% least strongly expressed genes were Receptor-interacting Serine/Threonine Kinase-2 (RIP-2), Complement component 3 (C3), Prostaglandin-endoperoxide synthase-2 (COX-2), Interleukin-8 (IL-8), Endothelin-1 (EDN-1), Plasminogen activator inhibitor type-2 (PAI-2), Matrix-metalloproteinase-14 (MMP-14), and Interferon regulating factor-7 (IRF-7). Gene expression profiles found in periodontal tissues following therapy indicate activation of pathways that regulate tissue damage and repair.
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影响因子：
7.6
作者：
QUIRYNEN, M;BOLLEN, CML;EYSSEN, H
通讯作者：
EYSSEN, H
影响因子：
6.4
作者：
Agenès, F;Bosco, N;Ceredig, R
通讯作者：
Ceredig, R
影响因子：
4.8
作者：
Kim, Young Sun;Cho, Kyung-Ok;Cho, Young-Jin
通讯作者：
Cho, Young-Jin
影响因子：
6.7
作者：
Cugini, MA;Haffajee, AD;Socransky, SS
通讯作者：
Socransky, SS
影响因子：
4.3
作者：
Hirose, M;Ishihara, K;Okuda, K
通讯作者：
Okuda, K