Influence of Core Oligosaccharide of Lipopolysaccharide to Outer Membrane Behavior of Escherichia coli.
Influence of Core Oligosaccharide of Lipopolysaccharide to Outer Membrane Behavior of Escherichia coli.
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DOI：
10.3390/md13063325
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发表时间：
2015-05-27
期刊：
影响因子：
5.4
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Wang Z;Wang J;Ren G;Li Y;Wang X
脂多糖是革兰氏阴性菌外膜的主要分子，对细胞膜的完整性起着重要作用。然而，脂多糖的核心低聚糖如何影响膜行为尚不清楚。本研究利用一系列影响脂多糖核心寡糖生物合成的基因缺陷型大肠杆菌突变株，研究了脂多糖核心寡糖与膜行为的关系。比较了这些突变体细胞的表面疏水性、外膜通透性、生物膜形成和自动聚集。与野生型W3110相比，突变体ΔwaaC、ΔwaaF、ΔwaaG、ΔwaaO、ΔwaaP、ΔwaaY和ΔwaaB的细胞表面疏水性增强，ΔwaaC、ΔwaaF、ΔwaaG和ΔwaaP的外膜通透性显著增加，ΔwaaC、ΔwaaF、ΔwaaG、ΔwaaO、ΔwaaR、ΔwaaP、ΔwaaQ和ΔwaaY的生物膜形成能力降低，ΔwaaC、ΔwaaF、ΔwaaG、ΔwaaO、ΔwaaR、ΔwaaU、ΔwaaP和ΔwaaY显著增强。这些结果为研究脂多糖核心寡糖对细菌细胞膜行为的影响提供了新的思路。
Lipopolysaccharides, major molecules in the outer membrane of Gram-negative bacteria, play important roles on membrane integrity of the cell. However, how the core oligosaccharide of lipopolysaccharide affect the membrane behavior is not well understood. In this study, the relationship between the core oligosaccharide of lipopolysaccharide and the membrane behavior was investigated using a series of Escherichia coli mutants defective in genes to affect the biosynthesis of core oligosaccharide of lipopolysaccharide. Cell surface hydrophobicity, outer membrane permeability, biofilm formation and auto-aggregation of these mutant cells were compared. Compared to the wild type W3110, cell surface hydrophobicities of mutant ΔwaaC, ΔwaaF, ΔwaaG, ΔwaaO, ΔwaaP, ΔwaaY and ΔwaaB were enhanced, outer membrane permeabilities of ΔwaaC, ΔwaaF, ΔwaaG and ΔwaaP were significantly increased, abilities of biofilm formation by ΔwaaC, ΔwaaF, ΔwaaG, ΔwaaO, ΔwaaR, ΔwaaP, ΔwaaQ and ΔwaaY decreased, and auto-aggregation abilities of ΔwaaC, ΔwaaF, ΔwaaG, ΔwaaO, ΔwaaR, ΔwaaU, ΔwaaP and ΔwaaY were strongly enhanced. These results give new insight into the influence of core oligosaccharide of lipopolysaccharide on bacterial cell membrane behavior.
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影响因子：
2.8
作者：
Genevaux, P;Bauda, P;Oudega, B
通讯作者：
Oudega, B
影响因子：
3.1
作者：
Bengoechea, JA;Brandenburg, K;Moriyón, I
通讯作者：
Moriyón, I
影响因子：
6.4
作者：
O'Hara, AM;Moran, AP;Orren, A
通讯作者：
Orren, A
影响因子：
5.1
作者：
Balestrino, Damien;Ghigo, Jean-Marc;Forestier, Christiane
通讯作者：
Forestier, Christiane
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL