An effector from the Huanglongbing-associated pathogen targets citrus proteases.
An effector from the Huanglongbing-associated pathogen targets citrus proteases.
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DOI：
10.1038/s41467-018-04140-9
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发表时间：
2018-04-30
影响因子：
16.6
通讯作者：
Ma W
中科院分区：
文献类型：
--
作者：
Clark K;Franco JY;Schwizer S;Pang Z;Hawara E;Liebrand TWH;Pagliaccia D;Zeng L;Gurung FB;Wang P;Shi J;Wang Y;Ancona V;van der Hoorn RAL;Wang N;Coaker G;Ma W
柑橘产业正面临着来自黄龙冰的前所未有的挑战。所有品种均可受亚洲假丝酵母相关细菌‘Candidatus Liberibacter asiaticus’(Clas)的侵染，目前尚无已知的抗性。为了制定有效的治疗策略，迫切需要深入了解HLB的发病机制。在这里，我们使用在所有Clas分离物中保守的SEC递送效应器1(SDE1)作为分子探针来了解Clas毒力。我们发现SDE1直接与柑橘木瓜蛋白酶样半胱氨酸蛋白酶(PLCP)相互作用并抑制其活性。PLCP是防御诱导的，并且在感染Clas的树中表现出增加的蛋白质积累，这表明在柑橘的防御反应中发挥了作用。我们分析了田间样本中的plcp活性，揭示了在感染期间数量增加但活性保持不变的特定成员。表达SDE1的转基因柑橘也表现出较低的PLCP活性。这些数据表明，SDE1抑制柑橘PLCP，这是一种增强植物防御反应的免疫相关蛋白。柑橘绿化病威胁着世界各地柑橘作物的产量，但对其致病机制却知之甚少。这里，克拉克等人。结果表明，从伴生菌中克隆的效应子能够抑制宿主植物类木瓜蛋白半胱氨酸蛋白酶的活性，提示其可能在发病机制中发挥作用。
The citrus industry is facing an unprecedented challenge from Huanglongbing (HLB). All cultivars can be affected by the HLB-associated bacterium ‘Candidatus Liberibacter asiaticus’ (CLas) and there is no known resistance. Insight into HLB pathogenesis is urgently needed in order to develop effective management strategies. Here, we use Sec-delivered effector 1 (SDE1), which is conserved in all CLas isolates, as a molecular probe to understand CLas virulence. We show that SDE1 directly interacts with citrus papain-like cysteine proteases (PLCPs) and inhibits protease activity. PLCPs are defense-inducible and exhibit increased protein accumulation in CLas-infected trees, suggesting a role in citrus defense responses. We analyzed PLCP activity in field samples, revealing specific members that increase in abundance but remain unchanged in activity during infection. SDE1-expressing transgenic citrus also exhibit reduced PLCP activity. These data demonstrate that SDE1 inhibits citrus PLCPs, which are immune-related proteases that enhance defense responses in plants. Greening disease threatens the productivity of citrus crops worldwide yet the pathosystem is poorly understood. Here, Clark et al. show that an effector cloned from the associated bacteria can suppress host plant papain-like cysteine proteases' activity, suggesting its probable role in pathogenesis.
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DOI：
10.1073/pnas.1202867109
发表时间：
2012-06-19
影响因子：
11.1
作者：
Lozano-Torres, Jose L.;Wilbers, Ruud H. P.;Smant, Geert
通讯作者：
Smant, Geert
影响因子：
14.9
作者：
Letunic I;Doerks T;Bork P
通讯作者：
Bork P
影响因子：
4.4
作者：
Cox, Juergen;Neuhauser, Nadin;Mann, Matthias
通讯作者：
Mann, Matthias
影响因子：
7.2
作者：
Hu, Chaoyang;Ham, Byung-Kook;Lucas, William J.
通讯作者：
Lucas, William J.
影响因子：
7.4
作者：
Kaschani, Farnusch;Shabab, Mohammed;van der Hoorn, Renier A. L.
通讯作者：
van der Hoorn, Renier A. L.