Analysis of QTL for Grain Size in a Rice Chromosome Segment Substitution Line Z1392 with Long Grains and Fine Mapping of qGL-6
Analysis of QTL for Grain Size in a Rice Chromosome Segment Substitution Line Z1392 with Long Grains and Fine Mapping of qGL-6
复制标题
水稻长粒染色体片段代换系Z1392粒级QTL分析及qGL-6精细定位
DOI：
10.1186/s12284-020-00399-z
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发表时间：
2020-05
期刊：
影响因子：
5.5
通讯作者：
Zhao Fangming
中科院分区：
文献类型：
--
作者：
Zhang Ting;Wang Shiming;Sun Shuangfei;Zhang Yi;Li Juan;You Jing;Su Tian;Chen Wenbo;Ling Yinghua;He Guanghua;Zhao Fangming
背景
米粒大小不仅影响水稻产量，也是影响稻米外观品质的重要因素。然而，晶粒尺寸的遗传机制尚不清楚。
结果
鉴定出水稻染色体片段替换系 Z1392，它包含三个替换片段并产生长度增加的籽粒。三个染色体片段分别位于1、5、6号染色体上，替换片段的平均长度为3.17 Mb。细胞学分析表明，Z1392 粒长增加的主要原因可能是颖片中的细胞扩张。使用 Nipponbare/Z1392 产生的次要 F2 群体鉴定了与颗粒大小相关的性状的七个数量性状基因座 (QTL)。 Z1392粒长遗传主要受qGL-5和qGL-6两个主要QTL控制。 qGL-6定位于6号染色体1.26 Mb区域，OsARF19可能是其候选基因。基于QTL作图，筛选出3个单片段替换系（S1、S2和S3）和2个双片段替换系（D1和D2），并利用3个单片段替换系进一步验证了qGL-5和qGL-6的作图准确性。 QTL加性效应和上位效应分析表明，‘细惠18’等位基因qGL-5和qGL-6的加性效应估计使Z1392粒长分别增加0.22和0.15毫米。此外，检测到qGL-5和qGL-6之间存在正上位相互作用，这表明qGL-5和qGL-6对于粒长的金字塔化产生了具有更长粒的新基因型。
结论
三段替换系Z1392的粒长遗传主要受qGL-5和qGL-6两个主要QTL控制。 qGL-6被发现位于6号染色体上1.26 Mb的区域，OsARF19可能是其候选基因。 qGL-5 和 qGL-6 之间的正上位相互作用导致更长的晶粒。本研究结果可用于促进qGL-5和qGL-6基因的克隆，有助于提高水稻产量。
Background
Grain size affects not only rice yield but is also an important element in quality of appearance. However, the mechanism for inheritance of grain size is unclear.
Results
A rice chromosome segment substitution line Z1392, which harbors three substitution segments and produces grains of increased length, was identified. The three chromosome segments were located on chromosomes 1, 5, and 6, and the average length of the substitution segment was 3.17 Mb. Cytological analysis indicates that the predominant cause of increased grain length in Z1392 could be cell expansion in the glumes. Seven quantitative trait loci (QTLs) for grain size related traits were identified using the secondary F2 population produced by Nipponbare/Z1392. The inheritance of grain length in Z1392 was mainly controlled by two major QTLs, qGL-5 and qGL-6. qGL-6 was localized on a 1.26 Mb region on chromosome 6, and OsARF19 may be its candidate gene. Based on QTL mapping, three single-segment substitution lines (S1, S2, and S3) and two double-segment substitution lines (D1 and D2) were selected, and the mapping accuracy for qGL-5 and qGL-6 was further verified using three single-segment substitution lines. Analysis of QTL additive and epistatic effects revealed that the additive effect of alleles qGL-5 and qGL-6 from ‘Xihui 18’ was estimated to increase grain length of Z1392 by 0.22 and 0.15 mm, respectively. In addition, a positive epistatic interaction between qGL-5 and qGL-6 was detected, which indicates that the pyramiding of qGL-5 and qGL-6 for grain length produces a novel genotype with longer grains.
Conclusions
Inheritance of grain length in the triple-segment substitution line Z1392 is mainly controlled by two major QTLs, qGL-5 and qGL-6. qGL-6 was found to be located in a 1.26 Mb region on chromosome 6, and OsARF19 may be its candidate gene. A positive epistatic interaction between qGL-5 and qGL-6 results in longer grains. The present results can be used to facilitate cloning of the qGL-5 and qGL-6 genes and contribute to improvement of grain yield in rice.
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