Multicomponent chemical imaging of pharmaceutical solid dosage forms with broadband CARS microscopy.
Multicomponent chemical imaging of pharmaceutical solid dosage forms with broadband CARS microscopy.
复制标题
具有宽带汽车显微镜的药物固体剂型形式的多组分化学成像。
DOI：
10.1021/ac400671p
复制
发表时间：
2013-09-03
影响因子：
7.4
通讯作者：
Cicerone, Marcus T.
中科院分区：
文献类型：
--
作者：
Hartshorn, Christopher M.;Lee, Young Jong;Camp, Charles H., Jr.;Liu, Zhen;Heddleston, John;Canfield, Nicole;Rhodes, Timothy A.;Walker, Angela R. Hight;Marsac, Patrick J.;Cicerone, Marcus T.
我们比较了相干拉曼成像模式，宽带相干反斯托克斯拉曼散射（BCARS）显微镜，与自发拉曼显微镜用于多组分药物的定量和定性评估。以吲哚美辛为模型活性药物成分（API），采用表格固体剂型，包埋在常用赋形剂中进行分析。与宽视场自发拉曼化学成像相比，BCARS获得的图像速度快10倍，具有更高的空间化学分辨率和更高的信噪比，从而消除了对化学成分识别的多元方法的需求。空间化学分辨率的显著增加使得自发宽场方法和体拉曼光谱无法识别出意想不到的API相。我们利用共聚焦自发拉曼光谱证实了意想不到的API相的存在，该方法提供了与BCARS相似的空间化学分辨率，但采集时间慢了100倍。
We compare a coherent Raman imaging modality, broadband coherent anti-Stokes Raman scattering (BCARS) microscopy, with spontaneous Raman microscopy for quantitative and qualitative assessment of multicomponent pharmaceuticals. Indomethacin was used as a model active pharmaceutical ingredient (API) and was analyzed in a tabulated solid dosage form, embedded within commonly used excipients. In comparison with wide-field spontaneous Raman chemical imaging, BCARS acquired images 10× faster, at higher spatiochemical resolution and with spectra of much higher SNR, eliminating the need for multivariate methods to identify chemical components. The significant increase in spatiochemical resolution allowed identification of an unanticipated API phase that was missed by the spontaneous wide-field method and bulk Raman spectroscopy. We confirmed the presence of the unanticipated API phase using confocal spontaneous Raman, which provided spatiochemical resolution similar to BCARS but at 100× slower acquisition times.
登录
查看更多内容
影响因子：
3.7
作者：
Lee, Young Jong;Parekh, Sapun H.;Cicerone, Marcus T.
通讯作者：
Cicerone, Marcus T.
影响因子：
3.7
作者：
Hancock, BC;Parks, M
通讯作者：
Parks, M
影响因子：
2.5
作者：
Garbacik, Erik T.;Herek, Jennifer L.;Offerhaus, Herman L.
通讯作者：
Offerhaus, Herman L.
影响因子：
3.6
作者：
Kee, TW;Cicerone, MT
通讯作者：
Cicerone, MT
影响因子：
3.3
作者：
Jung, Min-Sook;Kim, Jeong-Soo;Velaga, Sitaram P.
通讯作者：
Velaga, Sitaram P.