Effect of Warm Acupuncture Combined with Bone Marrow Mesenchymal Stem Cells Transplantation on Cartilage Tissue in Rabbit Knee Osteoarthritis.
Effect of Warm Acupuncture Combined with Bone Marrow Mesenchymal Stem Cells Transplantation on Cartilage Tissue in Rabbit Knee Osteoarthritis.
复制标题
温针灸联合骨髓间充质干细胞移植对兔膝骨关节炎软骨组织的影响
DOI：
10.1155/2021/5523726
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Ma YY
中科院分区：
文献类型：
--
作者：
Liu JW;Wu YL;Wei W;Zhang YL;Liu D;Ma XX;Li C;Ma YY
本研究旨在探讨温针灸联合骨髓间充质干细胞（BMSC）移植对兔膝骨关节炎（KOA）软骨组织损伤的影响及其机制。将50只家兔随机分为5组：空白组、膝骨关节炎组、温针灸组、骨髓基质细胞组、温针灸联合骨髓基质细胞组。采用改良Lequesne MG膝关节评定量表评价膝关节行为学程度，太平鹏法大体观察KOA兔软骨组织形态学变化，苏木精-伊红染色、番红O绿色染色、甲苯胺蓝染色评价软骨组织病理学程度。透射电镜和TUNEL染色观察细胞凋亡情况，免疫组化和qPCR检测凋亡相关蛋白和mRNA的表达。结果显示，温针灸联合骨髓间充质干细胞治疗可恢复关节功能，显著降低Lequesne MG评分。软骨组织病理损伤程度得到改善，软骨超微结构退变得到恢复，外周血管轻度水肿，血供逐渐恢复，甚至出现少量红细胞。此外，温针灸联合BMSCs治疗可通过减少TUNEL阳性软骨细胞抑制膝骨关节炎兔软骨细胞凋亡，同时逆转Bax、Bcl-2和Caspase-3的mRNA表达。提示温针灸联合骨髓间充质干细胞移植对兔膝骨性关节炎具有潜在的保护作用，其机制可能与抑制软骨细胞凋亡有关。
The current study was designed to investigate the effect and underlying mechanism of warm acupuncture combined with bone marrow mesenchymal stem cells (BMSC) transplantation on cartilage tissue injury in rabbit knee osteoarthritis (KOA). In the study, 50 rabbits were randomly divided into 5 groups: blank group, KOA group, warm acupuncture group, BMSCs group, and warm acupuncture combined with BMSCs group. After warm acupuncture combined with BMSCs, the Modified Lequesne MG knee joint assessment scale was used to evaluate the degree of knee joint behavior, the Taiping Peng method generally observed the histomorphology changes of KOA rabbit cartilage, and hematoxylin-eosin staining, safranin O green staining, and toluidine blue staining were conducted to evaluate the extent of cartilage tissue pathology. Furthermore, transmission electron microscopy and TUNEL staining were used to observe cell apoptosis, and immunohistochemistry and qPCR analysis were used to detect the expression of apoptosis-related proteins and mRNA. Results showed that administration of warm acupuncture combined with BMSCs recovered the joint function and significantly decreased Lequesne MG score. The degree of cartilage tissue pathological damage has been improved, cartilage ultrastructure degeneration has recovered, peripheral blood vessels have mild edema, blood supply has gradually recovered, and even small amounts of red blood cells have appeared. In addition, warm acupuncture combined with BMSCs treatment suppressed chondrocyte apoptosis in rabbits with knee osteoarthritis by reduced TUNEL-positive chondrocytes and simultaneously reversed the mRNA expression of Bax, Bcl-2, and Caspase-3. These results indicate that warm acupuncture combined with BMSCs transplantation has a potential protective effect on rabbit KOA, which may be mediated by inhibiting chondrocyte apoptosis.
登录
查看更多内容
影响因子：
--
作者：
Miao, Guiqiang;Zang, Xuehui;Zha, Zhengang
通讯作者：
Zha, Zhengang
通过 GSK3β-细胞周期蛋白 D1-CDK4/CDK6 信号通路针刀和人类脂肪间充质干细胞联合治疗膝骨关节炎兔的软骨保护作用
影响因子：
7.4
作者：
An X;Wang T;Zhang W;Yu H;Chunhua Zhao R;Guo Y;Wang C;Qin L;Guo C
通讯作者：
Guo C
影响因子：
2.6
作者：
Huang Y;Zhang Y;Ding X;Liu S;Sun T
通讯作者：
Sun T
影响因子：
1.8
作者：
Kim YS;Suh DS;Tak DH;Chung PK;Kwon YB;Kim TY;Koh YG
通讯作者：
Koh YG
影响因子：
2.7
作者：
Li Z;Wang J;Yang J
通讯作者：
Yang J